FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829226
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21120.25469534240363906No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20760.25035394452175885No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20020.24142995998678285No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19480.23491786316396254No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18610.22442615161608537No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17320.20886947587268126No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16820.2028397565922921No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16790.20247797343546872No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16140.19463933837096278No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14870.17932385139877427No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA14360.1731735377327773No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14250.1718469994910917No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14020.16907332862211266No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13980.16859095107968153No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13910.16774679038042706No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13910.16774679038042706No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13850.16702322406678033No Hit
GAGTATTAGTAGTAACTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCCAAGCTCCTGATCTATAAGGCGT13500.16280242057050792No Hit
GTAGTACACAGCCGTGTCCTCGGCTCTCAGGTAATTCATTTGTAGATACA13450.16219944864246902No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13350.16099350478639116No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT13220.15942577777348996No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13100.15797864514619658No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGTAA12220.1473663392127116No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12130.14628098974224157No Hit
GTGTACTACTGTGCGAGAGATTACTCCTACTATGGAGTGGGAAGTACCAC11960.14423088518690924No Hit
GAGTGAATTCTGCTCCAGATCCACGGCCGCTGAACCTTGATGGGACCCCA11900.1435073188732625No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11870.1431455357164392No Hit
CTACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGATGCAGACAGGGTGGAAGGAGA11780.14206018624596914No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11510.13880413783455897No Hit
CCTTATAGATCAGGAGCTTGGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAAGTTACTACTAATACTCTGA11280.13603046696557994No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTC11200.1350657118807177No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11130.1342215511814632No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11120.1341009567958554No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGACGGTGAGAGTGAATTCTGCTCCAGATCCACGGCCG10630.128191831901074No Hit
GAATTCACTCTCACCGTCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAATATAATAATTAT10580.1275888599730351No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10510.1267446992737806No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10340.12469459471844829No Hit
GTATTAGTAGTAACTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCC10340.12469459471844829No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC10240.12348865086237044No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10210.1231268677055471No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGA10200.12300627331993932No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG10130.12216211262068484No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10050.12119735753582256No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9990.12047379122217586No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9880.11914725298049025No Hit
GATCAGGAGCTTGGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAAGT9660.116494176497119No Hit
GGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG9370.11299693931449328No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC9200.11094683475916095No Hit
GGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC9140.11022326844551426No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9090.10962029651747533No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9060.10925851336065198No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAGGAGCTTGGGGGCTTTCCCTGGTTTCTG9010.10865554143261306No Hit
GATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGC8920.10757019196214301No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8900.10732900319092745No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8860.10684662564849631No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8830.10648484249167296No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGTAACTTGGCCTGGTATCAGCAGAAA8590.10359057723708615No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8450.10190225583857718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2050.043.2028161
GTATCAA18250.041.8097651
GCCGTTA350.001439268938.90417515
TATCAAC20200.037.5710142
TCAACGC21200.035.6318174
ATCAACG21350.035.2199753
CAACGCA21650.035.048375
AACGCAG21950.034.4143266
AGAGTAC23300.032.7124411
CGCAGAG23550.032.2187428
GCAGAGT24400.031.0982469
AAGTGCG553.5308942E-430.93343514
AGTACGG23350.028.41636813
GAGTACG24050.027.73076212
GTATTAC500.00822905827.2510091
GATGCGT500.00826548327.226352
AGCGACG500.00827278327.22142210
TCGTAGG659.371923E-426.1791822
TTCGGGG4750.025.92003670
TACGGGT1458.203642E-1025.82432215
Produced by FastQC (version 0.11.5)