FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382424
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38030.27509649716729456No Hit
CATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA36230.2620758898861709No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC27300.19747921043037447No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25600.18518197022042443No Hit
GCATAGATGAGGCGCCTAGGGGCTTCCCCTGGTTTCTGCTGATACCAGAC25550.18482028668483766No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC24160.17476548439552556No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23520.17013593514001493No Hit
CTGTAAGTCTGCTGACAATAGTAAGTCGCGAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC22280.16116618345746314No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22130.16008113285070283No Hit
GTGCAGCCACAGTTCCTTTGATCTCCACCATGGTCCCTCCGCCGAAAGCG21340.15436653298843192No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20750.15009866726850807No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT20740.15002633056139072No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT20540.14857959641904364No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20380.147422209105166No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20350.14720519898381396No Hit
GACTTACAGTTTCCCGCTCGCTTTCGGCGGAGGGACCATGGTGGAGATCAAAGGAACTGTGGCTGCACCAT19840.1435160269208289No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19370.1401162016863133No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT19320.13975451815072654No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19190.13881414095820097No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18920.13686104986603242No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18690.13519730560233328No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCACCATGGTCCCTCCGCCGAAAGCGAGCGGGAAA18010.13027840951835326No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16890.1221766983212097No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16360.11834285284398997No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16300.11790883260128586No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16160.1168961187016429No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTCGCGACTTACTATTGTCAGCAGACTTACAGTTTC15930.11523237443794379No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC15910.11508770102370908No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT15800.11429199724541819No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15700.11356863017424466No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15530.11233890615324965No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15360.11110918213225465No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15160.10966244798990758No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15110.10930076445432081No Hit
GCCTTAGAGTCGAGGACACGGCCGTATATTATTGTGCCAGCCTCTATGAC14910.10785403031197374No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA14700.10633495946250934No Hit
CCCCTAGGCGCCTCATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCA14610.10568392909845316No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14380.10402018483475403No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14270.10322448105646313No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14070.10177774691411606No Hit
GTAGGTGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGATGTTAGCAGATGGGTAGTCTGGTATCAGCAGAAA14010.10134372667141196No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13950.10090970642870783No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)