FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311756
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT9010.28900807041404175No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7210.23127060906606448No Hit
GTAATATACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGCTTCAGGGAGA6240.20015653267298786No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5570.1786653665045741No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5290.16968398362822207No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG5060.1623064191226472No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG4950.15877801870693747No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4730.15172121787551804No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4710.15107969052720718No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4610.14787205378565288No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT4290.13760761621267914No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC4090.13119234272957056No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT4050.12990928803294885No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG3890.12477706924646198No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG3880.12445630557230654No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT3790.12156943250490769No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT3650.11707874106673168No Hit
GGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGTAAAGCCCCTACCCTCCTGATCTATCTTTCATC3510.11258804962855566No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC3500.11226728595440023No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC3480.11162575860608939No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC3400.10905964921284594No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC3400.10905964921284594No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT3390.1087388855386905No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC3370.10809735819037965No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAA3350.1074558308420688No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA3320.10649353981960251No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAATTACTACTGGAGCTGGATACG3290.10553124879713621No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG3280.10521048512298078No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG3240.10392743042635909No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT3220.10328590307804822No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTTT100.00995138129.4249770
CTCGCTA208.7160704E-568.6583723
GACTACC200.007499764751.4937822
AGTACGC200.00750928751.4772578
GTATCAA5950.044.995751
TATTGAT3150.041.08729670
GCTTGCA603.0204865E-740.0442921
TCGCTAT350.001379558539.23335624
TAACGGC459.9689176E-538.35278336
TTAACGG459.987883E-538.34041235
TATCAAC7600.035.2269362
TTTAACG501.8598E-434.49524334
GTTTAAC501.8633284E-434.48412333
TATTAGA501.9151141E-434.323682
ATCAACG7950.033.6706623
TCAACGC8050.033.257734
CAACGCA8100.033.0524375
GTGGTAT751.7319871E-632.0354351
AACGCAG8400.031.8668776
GCTTGTA652.3986895E-531.6833951
Produced by FastQC (version 0.11.5)