FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311756
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8540.27393217772873657No Hit
GTAATATACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGCTTCAGGGAGA6430.206251042481941No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6160.1975904232797444No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC5900.18925056775170326No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5030.1613441281001809No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4490.14402288969578772No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4430.14209830765085515No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG4300.1379283798868346No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT4280.1372868525385237No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4270.13696608886436829No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT4100.131513106403726No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4080.13087157905541513No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4000.12830546966217168No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT3960.12702241496554997No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC3860.12381477822399568No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT3860.12381477822399568No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG3830.12285248720152941No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG3590.11515415902179911No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC3530.11322957697686652No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC3340.10713506716791338No Hit
GGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGTAAAGCCCCTACCCTCCTGATCTATCTTTCATC3300.10585201247129165No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC3300.10585201247129165No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAATTACTACTGGAGCTGGATACG3280.10521048512298078No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAA3260.10456895777466993No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA3260.10456895777466993No Hit
GGCTAACAGTTTCCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT3210.10296513940389279No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC3190.10232361205558194No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG3140.10071979368480477No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATTA150.002405403268.6246216
CGCCGAC200.00513732156.66077868
GTATAAG252.6197196E-454.9614371
ACGGTTA200.007085060252.23977750
ACGGGTA200.007514357651.46846416
GCAATAT200.007523951.4519513
GTATCAA6500.045.4488831
ACGGGTG704.656613E-1044.11582616
TACGGGT1300.042.2237615
GTATTAA350.001375274839.2581751
TATTGAT2700.039.1884670
TATCAAC7750.038.524522
TCAACGC7950.036.6684654
ATCAACG8150.036.1836243
GGGAACG1109.842386E-636.07119870
AACGCAG8250.034.924966
CAACGCA8350.034.917495
TTCATAC400.00267214934.29033
TGTTAGA1954.5110937E-1033.91309470
CAGAGTA8500.032.68712210
Produced by FastQC (version 0.11.5)