FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539000
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23870.44285714285714284No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17700.32838589981447125No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16310.30259740259740264No Hit
CTCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG14890.27625231910946196No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14170.26289424860853433No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG13850.25695732838589985No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13530.2510204081632653No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA13420.24897959183673468No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCACTCTCCACAGCTCCTGATCTATTTGGGTT12710.2358070500927644No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12580.23339517625231912No Hit
CTCCACAGCTCCTGATCTATTTGGGTTCTAGTCGGGCCTCCGGGGTCCCT12530.2324675324675325No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12070.22393320964749536No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA11880.2204081632653061No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11860.22003710575139146No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCACTCTC11700.21706864564007422No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11680.21669758812615955No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAATGAAGTTTG11520.2137291280148423No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG11240.2085343228200371No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10950.20315398886827457No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTA10400.19294990723562153No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG10270.19053803339517625No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT10210.18942486085343227No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10200.18923933209647495No Hit
GTCAGGGACCCCGGAGGCCCGACTAGAACCCAAATAGATCAGGAGCTGTGGAGAGTGCCCTGGCTTCTGCAGGTA9850.18274582560296845No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9540.1769944341372913No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9490.17606679035250464No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9430.17495361781076066No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9380.17402597402597403No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9150.16975881261595546No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACTC8920.16549165120593692No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA8790.16307977736549165No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA8610.15974025974025974No Hit
GTCTCCACTCTCCCTGCCCGTCACTCCTGGAGAGCCGGCCTCCATCTCCT8580.15918367346938775No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG8430.15640074211502783No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8420.15621521335807048No Hit
GCTGTGGAGAGTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATTACTATGCAGGAGGCTCTGA8070.149721706864564No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8030.1489795918367347No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7990.14823747680890537No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT7980.14805194805194805No Hit
TTATTACTGCATGCAAGCTCTGCAAACTTCATTCACTTTCGGCCCTGGGA7960.14768089053803338No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC7840.14545454545454545No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7810.14489795918367349No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA7480.13877551020408163No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7360.1365491651205937No Hit
GTTTATTACTGCATGCAAGCTCTGCAAACTTCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA7250.13450834879406307No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7190.13339517625231911No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7160.13283858998144712No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7150.1326530612244898No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7110.1319109461966605No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7050.13079777365491652No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC7040.1306122448979592No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG6770.12560296846011132No Hit
GCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC6750.12523191094619665No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6740.12504638218923933No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT6740.12504638218923933No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTGCAAACTTCATTCACTTTCGGCCCTGGGA6720.12467532467532468No Hit
GTATTACTGTGCGAGAGATGGTCGACGGTTCGGGGACTTATTATACCCCCTGGCCTTCGGTTTGGCCGTCTGGGG6620.1228200371057514No Hit
GTACCTGCAGAAGCCAGGGCACTCTCCACAGCTCCTGATCTATTTGGGTTCTAGTCGGGCCTCCGGGGTCCCTGA6210.11521335807050093No Hit
ATTTGGGTTCTAGTCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGT6140.11391465677179963No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6140.11391465677179963No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT6130.1137291280148423No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT6060.112430426716141No Hit
CTCCAGGAGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC6010.11150278293135436No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC5990.1111317254174397No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTGCAAAC5950.1103896103896104No Hit
CTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCACTCTCCACAGCTCCTGAT5940.11020408163265305No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC5860.10871985157699443No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC5820.10797773654916511No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC5790.10742115027829315No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT5770.10705009276437848No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCA5770.10705009276437848No Hit
GCAGAAGCCAGGGCACTCTCCACAGCTCCTGATCTATTTGGGTTCTAGTCGGGCCTCCGGGGTCCCTGACAGGTT5750.10667903525046384No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA5720.10612244897959183No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT5710.10593692022263451No Hit
AGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCACTCTCCACAG5680.10538033395176252No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5660.10500927643784785No Hit
GTAATACAGAGCCGTGTCCTCAGCTCTCAGGCTGTTCATATGCAGATACA5610.10408163265306122No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG5490.10185528756957328No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC5480.10166975881261596No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG5480.10166975881261596No Hit
ACTCTCCACAGCTCCTGATCTATTTGGGTTCTAGTCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGAT5460.1012987012987013No Hit
ATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGAGTGACGGGCAGGGAGAGTGGAGA5410.10037105751391465No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCACTCTCCACAGCTCCTGATCTATTTGGGTTCTAGT5410.10037105751391465No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTT100.009955275129.4244570
GAATTAG200.00741811151.6416933
TAGTCGC200.007467296451.5554549
GGTATCA4700.049.731861
TACTGTT608.6459925E-443.14148370
GTATCAA11950.041.708421
TATCAAC13650.036.7623752
ATCAACG14400.035.0830963
GAATAGA601.3753977E-534.3734931
TCAACGC14750.034.2506184
CAACGCA15000.033.9120335
AACGCAG15150.033.576276
CGCAGAG16100.031.5950648
CAGAGTA16200.031.4000310
AGAGTAC16450.031.13176311
CCCCCGT450.00448018430.86058846
ACGCAGA16650.030.551387
GAACGGG450.0047064530.551386
CAACGAG1104.7344842E-429.4146570
TGGTATC953.3056676E-728.9434132
Produced by FastQC (version 0.11.5)