FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences877089
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCCGGCTCACTCTCC29850.3403303427588306No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29370.3348576940310505No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACCTCTGGATGGTTAGCATTGGTATTG28760.3279028696061631No Hit
GTATTATGCAAGTTGGTACCAGCAGAAGCCAGGACAGGCCCCTCTACTTGTCATCTATGGTAAAAACAACCGTCC25910.29540901778496825No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACCTCTGGATGG25040.28548984196586663No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24400.27819297699549306No Hit
AGCCAGGACAGGCCCCTCTACTTGTCATCTATGGTAAAAACAACCGTCCC22690.2586966659027761No Hit
GTACAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTCCCCTGACCTGGAG22080.2517418414778888No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21510.2452430711136498No Hit
GACTATTACTGTACCTCCCGCAATACCAATGCTAACCATCCAGAGGTGAT20700.23600797638552076No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC20030.22836907086966088No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG19930.22722893571804No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19740.22506267892996035No Hit
TTACTGTACCTCCCGCAATACCAATGCTAACCATCCAGAGGTGATATTCG18850.21491547608053457No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18090.20625044892821598No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17970.2048822867462709No Hit
GTCCTGGCTTCTGCTGGTACCAACTTGCATAATACCTTCTGAGGCTGTCTGCTTGGCATGTGATCCTGACTGT17790.20283004347335332No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17750.20237398941270499No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17100.1949631109271693No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16920.19291086765425175No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGATTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA16900.19268284062392754No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16500.18812230001744407No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCAC15390.17546679983445237No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15380.1753527863192903No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15150.17273047547056228No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15130.1725024484402381No Hit
CTCTTTGCATAGGTTCTGTGATTTCTTCTGAGCTGACTCAGGACCCTGCT14610.16657374565180957No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14550.16588966456083704No Hit
GTAGAGGGGCCTGTCCTGGCTTCTGCTGGTACCAACTTGCATAATACCTT14160.16144313746951564No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13750.15676858334787006No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13750.15676858334787006No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGCAGACAGCCTCAGAAGGTATTATGCAAGTTGGTA13680.15597048874173544No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13530.15426028601430414No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13470.15357620492333163No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13440.15323416437784534No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13200.15049784001395525No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13130.14969974540782063No Hit
GGCCTGGACCCCTCTCCGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGATTTCTTCTGAGCTGACTCAGGA12770.14559525886198552No Hit
GCATAATACCTTCTGAGGCTGTCTGCTTGGCATGTGATCCTGACTGTCTG11490.13100152892123834No Hit
CTATTACTGTACCTCCCGCAATACCAATGCTAACCATCCAGAGGTGATATTCGGCGGAGGGACCAAGCTGACCGT11440.13043146134542788No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11330.12917731267864493No Hit
CCCCTCTACTTGTCATCTATGGTAAAAACAACCGTCCCTCAGGGATTCCA11230.12803717752702407No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11100.1265550018299169No Hit
ATCCAGAGGTGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT11050.12598493425410648No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11040.1258709207389444No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA11010.12552888019345812No Hit
CCTCTGGATGGTTAGCATTGGTATTGCGGGAGGTACAGTAATAGTCAGCC10900.12427473152667518No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTACCTCCCGC10790.12302058285989222No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10750.12256452879924386No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10620.12108235310213672No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACCTCTGGATGGT10410.11868806928373289No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9850.11230331243465601No Hit
GTCTCCACCATGGCCTGGACCCCTCTCCGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGATTTCTTCTGAG9790.11161923134368348No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9730.11093515025271095No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9550.10888290697979339No Hit
GCATTGGTATTGCGGGAGGTACAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG9420.10740073128268626No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9380.10694467722203789No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9260.10557651504009284No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9230.1052344744946066No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9220.1051204609794445No Hit
GGACAGGCCCCTCTACTTGTCATCTATGGTAAAAACAACCGTCCCTCAGG9160.10443637988847199No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC8950.10204209607006814No Hit
ATACCAATGCTAACCATCCAGAGGTGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTG8830.1006739338881231No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT8790.10021787982747475No Hit
GCCTCAGAAGGTATTATGCAAGTTGGTACCAGCAGAAGCCAGGACAGGCC8790.10021787982747475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8100.046.987831
GTGGTAT1950.038.684371
GTATCAA19800.038.098241
CTACGCT1250.035.67826528
TCGTATG1102.7284841E-1134.53457340
TATCAAC22100.034.437682
TCAACGC22800.033.380384
ATCAACG22700.033.3764083
CAACGCA23650.032.1806645
AACGCAG23800.031.9778426
CTCGTAT1207.6397555E-1131.63670739
TCTCGTA1207.8216544E-1131.59318738
CGCAGAG25350.030.2913388
ACGCAGA25250.030.1397747
TGGTATC2550.029.5771052
GCCGTCT1301.8371793E-1029.36660846
AGAGTAC26000.029.00666211
TACGGGT2500.028.79570415
CAGAGTA26900.028.4184910
CGTATGC1352.9831426E-1028.1716441
Produced by FastQC (version 0.11.5)