FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences877089
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCCGGCTCACTCTCC28070.32003593705997907No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28000.31923784245384446No Hit
GTATTATGCAAGTTGGTACCAGCAGAAGCCAGGACAGGCCCCTCTACTTGTCATCTATGGTAAAAACAACCGTCC27570.3143352613018747No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACCTCTGGATGGTTAGCATTGGTATTG24730.2819554229958419No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACCTCTGGATGG22630.25801258481180356No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22620.2578985712966415No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21970.25048769281110583No Hit
AGCCAGGACAGGCCCCTCTACTTGTCATCTATGGTAAAAACAACCGTCCC21850.24911953062916078No Hit
GACTATTACTGTACCTCCCGCAATACCAATGCTAACCATCCAGAGGTGAT20680.23577994935519656No Hit
GTACAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTCCCCTGACCTGGAG19910.22700090868771586No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18940.21594159771699337No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG18390.20967085438307856No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC17610.20077780020043576No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGATTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA17600.20066378668527365No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17560.20020773262462532No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17550.20009371910946322No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17400.19838351638203192No Hit
TTACTGTACCTCCCGCAATACCAATGCTAACCATCCAGAGGTGATATTCG17390.19826950286686984No Hit
GTCCTGGCTTCTGCTGGTACCAACTTGCATAATACCTTCTGAGGCTGTCTGCTTGGCATGTGATCCTGACTGT17080.19473508389684513No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16560.1888063811084166No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16090.1834477458957985No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15500.17672094850123535No Hit
CTCTTTGCATAGGTTCTGTGATTTCTTCTGAGCTGACTCAGGACCCTGCT15250.17387061062218315No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15110.17227442140991392No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14480.16509156995470242No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14250.16246925910597443No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCAC14180.16167116449983981No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14070.16041701583305684No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13910.15859279959046346No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13340.15209402922622448No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGCAGACAGCCTCAGAAGGTATTATGCAAGTTGGTA13260.15118192110492779No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12850.1465073669832822No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12820.14616532643779595No Hit
GTAGAGGGGCCTGTCCTGGCTTCTGCTGGTACCAACTTGCATAATACCTT12800.14593729940747174No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12690.1446831507406888No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12580.14342900207390585No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12250.13966655607355696No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11700.13339581273964216No Hit
CCCCTCTACTTGTCATCTATGGTAAAAACAACCGTCCCTCAGGGATTCCA11680.133167785709318No Hit
GGCCTGGACCCCTCTCCGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGATTTCTTCTGAGCTGACTCAGGA11620.13248370461834547No Hit
CTATTACTGTACCTCCCGCAATACCAATGCTAACCATCCAGAGGTGATATTCGGCGGAGGGACCAAGCTGACCGT11440.13043146134542788No Hit
GCATAATACCTTCTGAGGCTGTCTGCTTGGCATGTGATCCTGACTGTCTG11390.12986139376961744No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11170.12735309643605153No Hit
CCTCTGGATGGTTAGCATTGGTATTGCGGGAGGTACAGTAATAGTCAGCC11010.12552888019345812No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10850.12370466395086473No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10530.12005623146567794No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10060.11469759625305984No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9870.11253133946498017No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9830.11207528540433183No Hit
ATCCAGAGGTGATATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT9750.11116317728303513No Hit
GCATTGGTATTGCGGGAGGTACAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG9530.10865487994946921No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTACCTCCCGC9530.10865487994946921No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9440.10762875831301043No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA9410.10728671776752416No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9300.10603256910074121No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC9210.10500644746428242No Hit
GTCTCCACCATGGCCTGGACCCCTCTCCGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGATTTCTTCTGAG9170.10455039340363406No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9140.1042083528581478No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC9080.10352427176717528No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8830.1006739338881231No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACCTCTGGATGGT8810.10044590685779893No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8800.10033189334263683No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAG200.00734301751.7774640
GTGGTAT2400.042.8425181
TGGTATC2450.041.9346732
CGAAGTA350.001102844441.06892866
GGTATCA8500.040.7255941
GTATCAA22000.040.038281
AGTTAGC350.001398538439.132338
GTCTATA451.040879E-438.0822371
TATCAAC24500.035.9240382
ATCAACG24700.035.7677273
TCAACGC24900.035.478414
GATCGAC400.002326082435.2808359
CAACGCA25700.034.6424645
AACGCAG26100.033.9803476
CGCAGAG27050.032.7850848
AGAGTAC27250.032.4206511
CAGAGTA27400.032.2413310
ACGCAGA28300.031.5807657
GTTCCGC450.004773088330.4657921
AGTACGG25950.028.90179613
Produced by FastQC (version 0.11.5)