FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1390265
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34550.039.613911
GGTATCA15900.036.459651
TATCAAC39350.034.953552
CAACGCA41300.033.4656875
TCAACGC41600.033.306794
ATCAACG41500.033.3056073
AACGCAG43100.032.145316
TTACGCA450.004678356530.59215533
TCGTATG1950.030.17702340
ACGCAGA46100.030.0545047
CGCAGAG48000.028.8648478
AGAGTAC48750.028.56146811
CTCGTAT1700.028.47221639
CAGAGTA50050.027.75109310
GTGGTAT6050.027.2150251
GCAGAGT51050.027.2074859
GAGTACG37850.026.004312
AGTACGG38250.025.73328413
CGAATTA2000.025.7211515
CTTATAC3000.025.1549971
Produced by FastQC (version 0.11.5)