FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726708
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20280.27906669528889183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10000.043.2358971
GTATCAA23700.040.9390031
TATCAAC28200.034.5031432
TCAACGC28650.033.9495244
ATCAACG28550.033.827873
CAACGCA29400.033.0834665
CGTATCA651.9324028E-532.7094756
AACGCAG30650.032.1780556
ACGCAGA31600.030.9954327
AGAGTAC32750.029.49572811
CGCAGAG33400.029.2231988
CAGAGTA33400.029.22319810
GCAGAGT33800.028.7767439
ACGGGAT1800.026.46956316
AGTACGG26800.026.27166413
TAGCGGC550.00997214426.19745664
TACGGGG10400.025.84255815
GAGTACG27300.025.79049912
CGAACTA700.001433911224.3372724
GTATTAG1701.9826984E-1024.0310691
Produced by FastQC (version 0.11.5)