FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64199
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT16372.5498839545787315No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT5560.866057103693204No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT4680.7289833174971573Illumina PCR Primer Index 7 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3310.5155843548964937No Hit
CCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATAC2850.44393214847583296No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGG2680.4174519852334148No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1850.28816648234396175No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1790.2788205423760495No Hit
CCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATACC1710.2663592890854998No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.2616863191015436No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.2336484991978068No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.22586021589121327No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1360.21184130593934486No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.21028364927802612No Hit
GGTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGG1310.20405302263275127No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGT1280.19938005264879513No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGGCG1270.19782239598747647No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.19470708266483902No Hit
CGCCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATAC1240.1931494260035203No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.1931494260035203No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.18536114269692675No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.18380348603560803No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.17757285939033318No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.17134223274505833No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1090.16978457608373962No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.1682269194224209No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1060.16511160609978348No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.16199629277714606No Hit
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTG1020.15888097945450866RNA PCR Primer, Index 41 (96% over 27bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.15732332279318995No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT980.15265035280923378No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT920.14330441284132153No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.1401890995186841No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.13551612953472794No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.1277278462281344No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.11838190626022212No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT750.1168242495989034No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.11526659293758469No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT730.11370893627626599No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.10903596629230985No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.10124768298571628No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.10124768298571628No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)