EGAF00005761528 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005761528
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	64199
Sequences flagged as poor quality	0
Sequence length	20-76
%GC	52

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	4366	6.800728983317497	No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGG	282	0.43925917849187684	No Hit
CCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATAC	271	0.42212495521737103	No Hit
GGTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGG	169	0.26324397576286235	No Hit
CCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATACC	133	0.2071683359553887	No Hit
CGCCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATAC	125	0.19470708266483902	No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGGCG	119	0.18536114269692675	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	110	0.17134223274505833	No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAA	92	0.14330441284132153	Illumina RNA PCR Primer (96% over 31bp)
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC	91	0.14174675618000282	No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGGCGGGACAAAAA	90	0.1401890995186841	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	88	0.13707378619604665	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	78	0.12149721958285954	No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGGCGGGAGAAAAA	76	0.11838190626022212	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74	0.11526659293758469	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74	0.11526659293758469	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70	0.10903596629230985	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	68	0.10592065296967243	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	68	0.10592065296967243	No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGGCGGGATAAAAA	67	0.1043629963083537	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGACTT	15	0.0011522346	82.496185	53
GTGTTAT	15	0.0043726736	58.96259	1
CGGCACT	30	0.007465768	51.461906	69
TAACAAC	25	5.5370276E-4	47.17007	2
TCATTAA	40	8.723471E-4	42.982388	60
ATCATTA	40	9.071222E-4	42.64318	59
CGAGAGA	30	0.0011669741	40.54073	27
GCGAGAG	45	4.77327E-6	40.02593	26
GAATACA	30	0.0013585703	39.30839	1
CATTAAA	45	0.00148009	38.601353	61
TATCATT	45	0.0016460314	37.77728	58
GTATCAT	45	0.001673528	37.650368	57
AGTACGA	40	1.2498887E-4	36.851616	13
GCCGTAT	45	0.0018966525	36.704998	54
CGCCGTA	45	0.0019068622	36.66497	53
TCGGTGG	45	0.00243821	34.87736	45
TACAAAT	35	0.0028843984	33.71919	15
AGATCTC	45	0.0030507813	33.32188	40
TAGATCT	45	0.0032486767	32.89802	39
TGCGAGA	55	1.9518775E-5	32.593277	25