FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1188404
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55840.46987388127269847No Hit
GTCCCAACCAGGGCACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG54760.4607860626520947No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48020.40407134274203044No Hit
CCATGATACTCTCCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT46090.3878310742811367No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG41940.35291028976677963No Hit
GTATCATGGTGTTGACAGTAATATGTTGCAAGATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTC40960.3446639358332688No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36960.3110053483495512No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33300.2802077408019495No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32760.27566383149164764No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29970.25218696672175456No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29700.2499150120666036No Hit
GAGTATCATGGTGTTGACAGTAATATGTTGCAAGATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAAT29500.24823208269241773No Hit
GTAATATGTTGCAAGATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA29300.24654915331823185No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG28270.23788206704117454No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27420.23072961720088456No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27250.22929912723282653No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26070.21936984392512984No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25890.21785520748836257No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAGGTG24100.20279298958939887No Hit
GTCTCCGTCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAACAA23840.20060518140295724No Hit
CAGTAATATGTTGCAAGATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA23760.19993200965328287No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23690.19934298437231782No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23330.19631371149878324No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23300.19606127209265536No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC23100.19437834271846946No Hit
ATGATACTCTCCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA22720.19118077690751628No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22550.18975028693945828No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22550.18975028693945828No Hit
CTCCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG22340.1879832110965631No Hit
ATATTACTGTCAACACCATGATACTCTCCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGG21810.18352344825497052No Hit
GTGGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAACAACTATTTAATTTGGTATCAGCAAAAA21350.179652710694343No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21150.17796978132015712No Hit
ACATATTACTGTCAACACCATGATACTCTCCTCACCTTCGGCGGAGGGAC20960.17637099841468054No Hit
GGGCACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT20600.17334172554114594No Hit
GATCTACGATGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTA20150.1695551344492277No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCGTC19590.16484293220150723No Hit
GGTGAGGAGAGTATCATGGTGTTGACAGTAATATGTTGCAAGATCTTCAG19510.16416976045183287No Hit
AGTCAGGACATTAACAACTATTTAATTTGGTATCAGCAAAAACCAGGGAG19370.16299170988990277No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCGTCCTCC18630.156764871205415No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18600.15651243179928712No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18450.1552502347686477No Hit
CCCCTAAGCTCCTGATCTACGATGCATCCAATTTGGAAACAGGGGTCCCA18000.15146364367672946No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17950.151042911333183No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17760.1494441284277064No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17640.14843437080319488No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT17550.14767705258481123No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17340.14590997674191605No Hit
CCAGGGCACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT17250.1451526585235324No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16940.14254411799354427No Hit
GTCCCAACCAGGGCACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGG16920.1423758250561257No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16910.1422916785874164No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16230.13656971871518442No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCT16110.13555996109067286No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15760.1326148346858476No Hit
CATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATCTTG15480.13025873356198733No Hit
GGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCT14890.12529409190813898No Hit
GATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATCTTGCAACATATTACTGTCAACACCATGATACTCTC14520.12218067256589511No Hit
GTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAAGATCTTGCAACATATTA14490.12192823315976722No Hit
GCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCGTCCTCCCTGTCTGCATCTGTGGGAGA14490.12192823315976722No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14460.12167579375363934No Hit
ACCTTGATGGGACCCCTGTTTCCAAATTGGATGCATCGTAGATCAGGAGCTTAGGGGCTCTCCCTGGTTTTTG14350.1207501825978371No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT14350.1207501825978371No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14190.1194038390984884No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14060.11830993500526756No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14030.1180574955991397No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13990.1177209097243025No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT13940.11730017738075604No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13850.1165428591623724No Hit
GCTTAGGGGCTCTCCCTGGTTTTTGCTGATACCAAATTAAATAGTTGTTA13820.11629041975624452No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGT13770.11586968741269803No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13340.11225138925819839No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC13320.1120830963207798No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13260.11157821750852404No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13230.11132577810239615No Hit
GTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCAGCAGCC13200.11107333869626826No Hit
TCGTAGATCAGGAGCTTAGGGGCTCTCCCTGGTTTTTGCTGATACCAAAT13030.10964284872821027No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12900.10854894463498946No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12900.10854894463498946No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12780.10753918701047792No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12660.10652942938596639No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12660.10652942938596639No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC12390.10425747473081545No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12390.10425747473081545No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12380.10417332826210615No Hit
CTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGTTGACCCAGTC12310.1035843029811411No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12290.1034160100437225No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12270.10324771710630391No Hit
GGCTCTCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCGTCCTCC12250.10307942416888533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19450.040.2410771
GTGGTAT2050.040.1894261
GCTAATC1550.039.9982833
TCGTATG1400.039.53698740
TCGCTAA1500.039.00242231
AATCTCG1450.038.0574436
CTTATAC1600.036.4739951
CGCTAAT1550.035.5435232
TATCAAC22500.034.9372832
CTCGCTA1700.034.39359330
TACGACA400.00266170734.32557712
CAACGCA23550.034.108385
TCAACGC23200.034.0310974
AACGCAG24050.033.3964546
ATCAACG23750.033.243013
ACGAGAC1800.032.42815422
ACTCGCT1850.031.59426529
CGTATGC1800.030.76925341
CGCAGAG26000.030.7597058
CTCGTAT1800.030.72883439
Produced by FastQC (version 0.11.5)