FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695024
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACAGCTTCCCCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA46810.673501922235779No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26830.38602983494095167No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22850.3287656253596998No Hit
GCTGTATACTTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAAT21690.31207555422546557No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTT19650.28272404981698473No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19600.2820046501991298No Hit
GTAATATACAGCCGTGTCTGCGGCGGTGACAGAACTCAGCTTCAGAGAGAACTGGTTCTTGGACGTGTCTACGGA19270.2772566127212873No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17800.2561062639563526No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17180.24718570869495154No Hit
GTCCAGGGGTGACGGACAGAGAGAGTGGAGTCTGGGTCATCACAATGTCT17050.24531526968852874No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16230.23351711595570804No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16160.23250995649071113No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG16100.2316466769492852No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC15940.22934459817214944No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGACATTGTGATGA15910.2289129584014365No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15230.21912912359860953No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15010.21596376528004788No Hit
GTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGAGACAGATT14750.21222288726720226No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14160.2037339717765142No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13790.19841041460438774No Hit
GGATACCTGGATCCAGTGCAGACATTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCTGGACAGCCGG13570.19524505628582609No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA13270.19092865857869656No Hit
GTGGAGGCTGGCCTGGCTTCTGTAGGTACCAATAGAAATAGGTCTTTCCA13180.18963373926655772No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12520.18013766431087272No Hit
GTATATTACTGTGCGAGACACGGTGGCTACGATTACTTTGGCCTGGTATT12340.177547825686595No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12250.17625290637445615No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12200.1755335067566012No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12050.17337530790303646No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11710.16848339050162298No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11510.16560579203020329No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG11490.16531803218306132No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGACATTGTGATGACCCAGACTCCACT11370.1635914731002095No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11270.16215267386449964No Hit
GTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT11260.16200879394092865No Hit
CCAGAGAACCGGTTGGAAACCTCATAGATCAGGAGCTGTGGAGGCTGGCC11250.16186491401735767No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11120.1599944750109349No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGACATTGTGATGACCCAGACTCCACTCTCT10920.15711687653951517No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10890.15668523676880222No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGGGGGGAAG10860.15625359699808927No Hit
CCTGGATCCAGTGCAGACATTGTGATGACCCAGACTCCACTCTCTCTGTC10630.15294435875595663No Hit
GCTAATGCTCTGGATACCTGGATCCAGTGCAGACATTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCC10580.15222495913810172No Hit
GATCAGGAGCTGTGGAGGCTGGCCTGGCTTCTGTAGGTACCAATAGAAAT10570.15208107921453073No Hit
CTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTA10550.15179331936738874No Hit
AGCCTCCACAGCTCCTGATCTATGAGGTTTCCAACCGGTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGT10540.15164943944381776No Hit
GTGCAGACATTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCT10240.14733304173668824No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10110.14546260273026543No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10050.14459932318883953No Hit
CTACAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAGGTTTCCAA9880.1421533644881328No Hit
GACTAGACTTGCAGGAGATGGAGGCCGGCTGTCCAGGGGTGACGGACAGA9310.13395220884458667No Hit
AGTCTAGTCAGAGCCTCCTTCATAATCATGGAAAGACCTATTTCTATTGGTACCTACAGAAGCCAGGCCAGCCTC9240.13294504937958976No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9190.13222564976173484No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9140.13150625014387993No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAATCTGTCTCTGACCCGCTGCCA9080.130642970602454No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9070.13049909067888302No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8930.12848477174888925No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8880.1277653721310343No Hit
GTATTAAAGGGGTTCGACTCCTGGGGCCAGGGAACCCTGGTCACCGTCTC8660.12460001381247267No Hit
GTTTATTACTGCATGCAAAGTATACAGCTTCCCCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA8640.1243122539653307No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8640.1243122539653307No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8600.12373673427104676No Hit
ACAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAGGTTTCCAACCGGTTCTCTGGAGTGCCAGATAGGTT8600.12373673427104676No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8570.1233050945003338No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8510.1224418149589079No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8420.12114689564676903No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT8420.12114689564676903No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8250.11870093694606229No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8130.11697437786321048No Hit
GTTCAGTGGCAGCGGGTCAGAGACAGATTTCACACTGAAAATCAGCCGGG8020.11539169870392965No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8000.11510393885678769No Hit
ATCTGGCACTCCAGAGAACCGGTTGGAAACCTCATAGATCAGGAGCTGTGGAGGCTGGCCTGGCTTCTGTAGGTA7930.11409677939179079No Hit
GCTCCTGATCTATGAGGTTTCCAACCGGTTCTCTGGAGTGCCAGATAGGT7910.11380901954464882No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7830.11265798015608094No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7650.11006814153180322No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT7590.10920486199037731No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7570.10891710214323534No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7520.10819770252538043No Hit
ATCATGGAAAGACCTATTTCTATTGGTACCTACAGAAGCCAGGCCAGCCTCCACAGCTCCTGATCTATGAGGTTT7490.10776606275466746No Hit
TTATTACTGCATGCAAAGTATACAGCTTCCCCCCACTTTCGGCGGAGGGA7460.10733442298395451No Hit
CCTCATAGATCAGGAGCTGTGGAGGCTGGCCTGGCTTCTGTAGGTACCAA7400.1064711434425286No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGACATTGTGATGACCCAGACTC7400.1064711434425286No Hit
GTGCCAGATAGGTTCAGTGGCAGCGGGTCAGAGACAGATTTCACACTGAA7400.1064711434425286No Hit
CTTCTGTAGGTACCAATAGAAATAGGTCTTTCCATGATTATGAAGGAGGC7400.1064711434425286No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7340.1056078639011027No Hit
TTCCATGATTATGAAGGAGGCTCTGACTAGACTTGCAGGAGATGGAGGCC7320.10532010405396072No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7320.10532010405396072No Hit
GGCTTCTGTAGGTACCAATAGAAATAGGTCTTTCCATGATTATGAAGGAG7300.10503234420681876No Hit
CCACTCTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTTCATAAT7260.10445682451253482No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7250.10431294458896384No Hit
GTATACTTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAAT7190.10344966504753793No Hit
GTCTCTGGTGCCTCCATCAACAGTCACAGTGACTACTGGGGCTGGATCCG7100.10215474573539907No Hit
TCTATGAGGTTTCCAACCGGTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGAGACAGATTTCACA7070.10172310596468612No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC7050.10143534611754414No Hit
CTCCAGAGAACCGGTTGGAAACCTCATAGATCAGGAGCTGTGGAGGCTGG7020.10100370634683119No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)