FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695024
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACAGCTTCCCCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA45410.6533587329358411No Hit
GCTGTATACTTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAAT30820.4434379244457745No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25740.37034692327171437No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18810.270638136237022No Hit
GTAATATACAGCCGTGTCTGCGGCGGTGACAGAACTCAGCTTCAGAGAGAACTGGTTCTTGGACGTGTCTACGGA18170.261429821128479No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17600.2532286654849329No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17010.2447397499942448No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAATCTGTCTCTGACCCGCTGCCA16490.2372579939685536No Hit
GTCCAGGGGTGACGGACAGAGAGAGTGGAGTCTGGGTCATCACAATGTCT16400.23596307465641475No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16010.23035175763714633No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15990.23006399779000436No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15760.22675475954787172No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC15610.22459656069430695No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14810.21308616680862819No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGACATTGTGATGA14790.21279840696148625No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14290.20560441078293698No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13830.19898593429867167No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13480.19395013697368724No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG13330.1917919381201225No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13080.18819494003084786No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG12830.18459794194157325No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12740.18330302262943438No Hit
GTGGAGGCTGGCCTGGCTTCTGTAGGTACCAATAGAAATAGGTCTTTCCA12480.17956214461658876No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12020.17294366813232348No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11950.1719365086673266No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGGGGGGAAG11830.17020994958447477No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTT11760.1692027901194779No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11630.16733235111305508No Hit
GGATACCTGGATCCAGTGCAGACATTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCTGGACAGCCGG11620.1671884711894841No Hit
GTATATTACTGTGCGAGACACGGTGGCTACGATTACTTTGGCCTGGTATT11460.16488639241234834No Hit
GTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGTCAGAGACAGATT11390.16387923294735146No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA11370.1635914731002095No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11270.16215267386449964No Hit
GTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT11220.16143327424664472No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10930.15726075646308618No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10800.15539031745666337No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10790.1552464375330924No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10190.14661364211883332No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGACATTGTGATGACCCAGACTCCACT10150.14603812242454936No Hit
CAGTAATAAACTCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAATCTGTCTCTGACCCGCTGCCA9930.1428727641059877No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGACATTGTGATGACCCAGACTCCACTCTCT9850.14172172471741984No Hit
CCAGAGAACCGGTTGGAAACCTCATAGATCAGGAGCTGTGGAGGCTGGCC9830.14143396487027787No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9800.1410023250995649No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9650.13884412624600015No Hit
GTATACTTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACCCGGCTGATTTTCAGTGTGAAAT9400.13524712815672552No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9380.13495936830958355No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9270.13337668915030274No Hit
GATCAGGAGCTGTGGAGGCTGGCCTGGCTTCTGTAGGTACCAATAGAAAT9250.13308892930316074No Hit
CCTGGATCCAGTGCAGACATTGTGATGACCCAGACTCCACTCTCTCTGTC9250.13308892930316074No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9210.1325134096088768No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9210.1325134096088768No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9170.13193788991459288No Hit
GACTAGACTTGCAGGAGATGGAGGCCGGCTGTCCAGGGGTGACGGACAGA8910.1281970119017473No Hit
GTCTCTGACCCGCTGCCACTGAACCTATCTGGCACTCCAGAGAACCGGTT8820.1269020925896084No Hit
GTTTATTACTGCATGCAAAGTATACAGCTTCCCCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA8780.12632657289532448No Hit
CTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTA8640.1243122539653307No Hit
GTGCAGACATTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCCCT8590.12359285434747577No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8580.12344897442390479No Hit
GCTAATGCTCTGGATACCTGGATCCAGTGCAGACATTGTGATGACCCAGACTCCACTCTCTCTGTCCGTCACCC8490.12215405511176593No Hit
GTATTAAAGGGGTTCGACTCCTGGGGCCAGGGAACCCTGGTCACCGTCTC8490.12215405511176593No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8420.12114689564676903No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8300.11942033656391722No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8270.11898869679320427No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8110.11668661801606851No Hit
ATCTGGCACTCCAGAGAACCGGTTGGAAACCTCATAGATCAGGAGCTGTGGAGGCTGGCCTGGCTTCTGTAGGTA8060.11596721839821358No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTTCATAATCATGGAAAGACCTATTT8050.1158233384746426No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7670.11035590137894519No Hit
GGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAAGTATACAGCTTCCCCCCACTTTCGGCGGAGGGAC7650.11006814153180322No Hit
GCCTCCACCCGGCTGATTTTCAGTGTGAAATCTGTCTCTGACCCGCTGCC7580.10906098206680633No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7580.10906098206680633No Hit
TTATTACTGCATGCAAAGTATACAGCTTCCCCCCACTTTCGGCGGAGGGA7580.10906098206680633No Hit
GTCTCTGGTGCCTCCATCAACAGTCACAGTGACTACTGGGGCTGGATCCG7540.10848546237252239No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7540.10848546237252239No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7410.10661502336609958No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7210.1037374248946799No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7160.10301802527682498No Hit
AGCCTCCACAGCTCCTGATCTATGAGGTTTCCAACCGGTTCTCTGGAGTGCCAGATAGGTTCAGTGGCAGCGGGT7160.10301802527682498No Hit
GGTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCAC7140.102730265429683No Hit
GGCTTCTGTAGGTACCAATAGAAATAGGTCTTTCCATGATTATGAAGGAG7070.10172310596468612No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGATCCAGTGCAGACATTGTGATGACCCAGACTC7040.10129146619397315No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT6960.10014042680540527No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)