FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659956
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22900.039.654121
GGTATCA9250.036.675111
TATCAAC25650.035.400022
ATCAACG26500.034.5231483
TCAACGC26600.034.264554
CAACGCA26650.034.2002645
AACGCAG27100.033.6247186
ACGCAGA28250.032.2559247
CGCAGAG28800.031.6399258
AGAGTAC29350.030.93029611
GTGGTAT3800.030.660081
TCGTATG802.6544312E-630.3378340
CAGAGTA30300.029.96053510
GAGTACG20050.029.38734612
GCAGAGT31500.029.0366849
AGTACGG20350.028.7857813
TGGTATC4250.028.2178672
CCCGACG500.00746574427.79926142
TACGGGA8900.026.56062715
GTACGGG22500.025.57838214
Produced by FastQC (version 0.11.5)