FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659956
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8700.13182697028286736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19800.040.0161441
TCACGAT700.002361897435.16581770
CAACGCA22800.034.5401155
ATCAACG22800.034.3951573
TATCAAC23050.034.181042
TCAACGC22950.034.1703534
AACGCAG23250.033.7243356
ACGCAGA24300.032.267117
GGTATCA8900.031.9872251
CGCAGAG24800.031.752228
AGAGTAC25050.031.7158911
CAGAGTA26050.030.49145710
GCAGAGT26350.030.1443069
GAGTACG20950.028.76903512
GTGGTAT3350.028.6682821
AGTACGG21100.028.4022213
TACGGGT3100.026.51423615
CGAATTA659.032278E-426.34427315
GTACGGG22800.024.93273414
TACGGGA9700.024.00859615
Produced by FastQC (version 0.11.5)