FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59721
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT10351.7330587230622394No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT2750.4604745399440733Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTCT2170.3633562733376869Illumina PCR Primer Index 11 (95% over 24bp)
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGG1690.2829825354565396No Hit
CCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATAC1670.27963362971149175No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.21432996768305954No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.20595770332044006No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.19758543895782052No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.19088762746772492No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130.18921317459520104No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1090.1825153631051054No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.1808409102325815No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.1707941929974381No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1010.16911974012491418No Hit
CCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATACC1000.16744528725239027No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.15739857001724686No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT910.15237521139967514No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.14735185278210344No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.14735185278210344No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT870.14567739990957954No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.14400294703705566No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.14232849416453175No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.13897958841948393No Hit
GTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGGCG790.1322817769293883No Hit
GGTATCAACGCAGAGTACGGGTGCCTGCGCAGAGAAATGG780.13060732405686443No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.1255839654392927No Hit
CGCCATTTCTCTGCGCAGGCACCCGTACTCTGCGTTGATAC740.1239095125667688No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.10549053096900589No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.10549053096900589No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.10214162522395809No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT600.10046717235143417No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTGT100.006750336147.1099770
GTAAATT158.443105E-489.1911966
TGTGGTT150.001148977582.5459853
ATGGGGG300.001806268573.5549870
AGGGGGG2600.073.5549870
GTAGGTC200.002684620266.6419265
GGTAGGT200.002820730265.8171464
TTGAACC200.003465423162.4914855
TATAGGC200.003596522161.9094953
AACGAAA200.004462322758.63313346
AAATGGG250.005523165755.5407368
AGGTCCG250.00605585454.26556467
ATATCAA451.6025297E-952.8318861
ACGGTAG250.007027453352.26560262
TACGGTA250.007130615452.07368561
GCATCTG200.00750037651.43157635
AGTATCA359.061878E-750.9450341
GCTTGAA2400.048.89174356
ATAGGTC200.00927506348.74450332
AAATATA250.00939783848.56643750
Produced by FastQC (version 0.11.5)