FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736487
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAACACTTTCCCCTTCACTTTCGGCCCTGGGACCAAGGTGGGCATCAAACGAACTGTGGCTGCACCAT36280.49260883084154916No Hit
GTGTTACCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCC30170.4096474207962938No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25580.34732452847097095No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20470.2779410906098818No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17380.23598515656080826No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17240.23408424045502502No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17030.23123286629635012No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15640.21235948496035909No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15200.206385177199326No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14940.20285490443144277No Hit
CCTTGGTCCCAGGGCCGAAAGTGAAGGGGAAAGTGTTACCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGG14060.19090628890937655No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13060.17732831672521035No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13010.17664941811600204No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12840.1743411628446938No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12740.1729833656262772No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT12590.17094666979865225No Hit
GTGCAGCCACAGTTCGTTTGATGCCCACCTTGGTCCCAGGGCCGAAAGTG12240.16619437953419408No Hit
CTCCTGATCTATGCTGCTTCCATTTTGCCAAGTGGGGTCTCGTCAAGGTT11980.16266410676631088No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11770.15981273260763598No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11720.15913383399842768No Hit
GATCTATGCTGCTTCCATTTTGCCAAGTGGGGTCTCGTCAAGGTTCAGCGGCAGTGGGTCTGGGGCAGATTTCA11640.15804759622369438No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11250.15275218707186958No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11150.15139438985345297No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11060.150172372356878No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGCCCACCTTGGTCC11040.14990081291319468No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11040.14990081291319468No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10990.14922191430398635No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGCTTCTGTTGATACCAGGCTAACC10730.14569164153610314No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10460.14202558904637827No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10250.13917421488770337No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC10200.13849531627849507No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10110.13727329878192013No Hit
GGTATCAACAGAAGCCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCTTCCATTTTGCCAAGTGGGGTCTCGT9940.13496504351061187No Hit
GCATAGATCAGGAGTTTAGGGGCTTTCCCTGGCTTCTGTTGATACCAGGC9760.13252100851746196No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTAACACTTTCCCCTTCACTTT9110.12369532659775392No Hit
GTCTCCATCTTCCGTGTCTGCATTTGTTGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGAATATTAACAA9090.12342376715407061No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9010.12233752937933731No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8950.12152285104828735No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8820.11975771466434573No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8810.11962193494250409No Hit
GGACCAAGGTGGGCATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC8710.11826413772408745No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8670.1177210188367208No Hit
ATTGTCAACAGGGTAACACTTTCCCCTTCACTTTCGGCCCTGGGACCAAGGTGGGCATCAAACGAACTGTGGCTG8640.11731367967119583No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8590.11663478106198752No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8310.11283294885042099No Hit
CGTCAAGGTTCAGCGGCAGTGGGTCTGGGGCAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTG8170.11093203274463771No Hit
GTTTAGGGGCTTTCCCTGGCTTCTGTTGATACCAGGCTAACCAGTTGTTA8090.10984579496990442No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8010.10875955719517112No Hit
ACCTTGACGAGACCCCACTTGGCAAAATGGAAGCAGCATAGATCAGGAGT7930.10767331942043784No Hit
GTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCA7920.10753753969859618No Hit
GTTGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGAATATTAACAACTGGTTAGCCTGGTATCAACAGAAG7810.1060439627583379No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7660.10400726693071297No Hit
GAATATTAACAACTGGTTAGCCTGGTATCAACAGAAGCCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCTTC7490.1016990116594047No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC7490.1016990116594047No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7380.10020543471914645No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTAACACTTTC7380.10020543471914645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2150.044.736751
CGTATGC801.5916157E-938.93091641
GACAGGT1850.035.279851
CCGGTAG400.002502775534.75745447
ATCAACG18200.033.587333
TCAACGC18350.033.312784
CAACGCA18500.033.042675
AACGCAG18750.032.6021046
TAGGCAA1252.6256474E-531.2698970
AATGGTA1901.5344995E-830.85844670
TCGTATG901.9468098E-730.74971240
CGCAGAG20700.029.5288858
AGAGTAC20850.029.31644811
CAGAGTA21000.029.27056910
ACGCAGA21250.028.7665627
GCAGAGT21800.028.1964179
ATCTCCG1103.8287908E-828.09617610
ACGGGGA3950.027.82523216
GCTTACC500.00791080827.4718179
GCGGTAC1155.3505573E-827.14366344
Produced by FastQC (version 0.11.5)