FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736487
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAACACTTTCCCCTTCACTTTCGGCCCTGGGACCAAGGTGGGCATCAAACGAACTGTGGCTGCACCAT37610.5106675338464902No Hit
GTGTTACCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCC32820.44562904708433415No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25250.3428437976501961No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16880.22919617046872515No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16670.22634479631005025No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15950.21656865633745062No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15260.20719985553037598No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14740.20013930999460955No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14610.19837417361066792No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13330.18099436921493522No Hit
CCTTGGTCCCAGGGCCGAAAGTGAAGGGGAAAGTGTTACCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGG13020.17678519783784372No Hit
CTCCTGATCTATGCTGCTTCCATTTTGCCAAGTGGGGTCTCGTCAAGGTT12380.16809529563997735No Hit
GATCTATGCTGCTTCCATTTTGCCAAGTGGGGTCTCGTCAAGGTTCAGCGGCAGTGGGTCTGGGGCAGATTTCA12210.1657870403686691No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12170.16524392148130246No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11780.15994851232947763No Hit
GTGCAGCCACAGTTCGTTTGATGCCCACCTTGGTCCCAGGGCCGAAAGTG11510.15628245983975278No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11500.1561466801179111No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11240.1526164073500279No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11170.15166594929713628No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11120.15098705068792795No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11100.15071549124424463No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT11060.150172372356878No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11030.149765033191353No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11030.149765033191353No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10990.14922191430398635No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10360.14066779182796166No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGCTTCTGTTGATACCAGGCTAACC10010.13591550156350352No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9910.13455770434508688No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9870.13401458545772021No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9830.13347146657035358No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9360.12708981964379548No Hit
GGTATCAACAGAAGCCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCTTCCATTTTGCCAAGTGGGGTCTCGT9320.12654670075642882No Hit
ATTGTCAACAGGGTAACACTTTCCCCTTCACTTTCGGCCCTGGGACCAAGGTGGGCATCAAACGAACTGTGGCTG9190.12478156437248722No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGCCCACCTTGGTCC8970.12179441049197066No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC8920.12111551188276236No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8850.12016505382987074No Hit
GGACCAAGGTGGGCATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC8830.1198934943861874No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8780.1192145957769791No Hit
GCATAGATCAGGAGTTTAGGGGCTTTCCCTGGCTTCTGTTGATACCAGGC8770.11907881605513743No Hit
GTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCA8730.11853569716777078No Hit
GTCTCCATCTTCCGTGTCTGCATTTGTTGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGAATATTAACAA8550.11609166217462087No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTAACACTTTCCCCTTCACTTT8530.11582010273093754No Hit
GTTTAGGGGCTTTCCCTGGCTTCTGTTGATACCAGGCTAACCAGTTGTTA8400.11405496634699594No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8330.11310450829410432No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8300.11269716912857931No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8250.11201827051937102No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8180.11106781246647938No Hit
CGTCAAGGTTCAGCGGCAGTGGGTCTGGGGCAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTG8010.10875955719517112No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7700.10455038581807961No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTAACACTTTC7660.10400726693071297No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7600.103192588599663No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7570.102785249434138No Hit
GGGGAAAGTGTTACCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCT7500.10183479138124638No Hit
GTTGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGAATATTAACAACTGGTTAGCCTGGTATCAACAGAAG7460.10129167249387974No Hit
GAGTGAAATCTGCCCCAGACCCACTGCCGCTGAACCTTGACGAGACCCCA7450.10115589277203807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGGA655.5334385E-737.0797834
TAATTCG655.5868986E-737.034332
ATCAACG19200.036.233283
ACGGGTA1151.8189894E-1235.7744716
TCAACGC19500.035.6782654
CAACGCA19650.035.2362825
AACGCAG19700.034.9681056
TTCGGAT709.824998E-734.4570935
CTAGAAC601.4048172E-534.2698973
TACGGGT2600.032.96077715
CGCAGAG21100.032.8103758
AGAGTAC21400.032.51277511
GTGGTAT1800.032.401221
AATTCGG751.702474E-632.1160933
CAGAGTA21950.031.693810
ACGCAGA21950.031.5398147
GCAGAGT22100.031.3236189
TCTTCGG1752.0339758E-730.5888470
GCCAGTT900.005367252529.73915170
TGTTAGA5250.029.31430470
Produced by FastQC (version 0.11.5)