FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1138423
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTCTTCATTTGCAGAAACA19420.1705868556766685No Hit
GTGTATTACTGTGCGAAGGCGGGGGTCGTTCGAGGTTCGGGGAGTTATTGGTCAAGGAACGCTGGTGCGAATTA14810.13009224163601754No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13950.122537931858369No Hit
CTCCAGCCCCGTCCCTGGAGCCTGGCGGACCCAGCCCATCCAATAGTTTC12520.10997669583274407No Hit
GTGTTGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCCACTGAACCT12230.10742931230307189No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11780.10347647579151159No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGC150.002371365468.883583
GTATCAA21700.039.0446171
GTGGTAT4150.034.856761
TATCAAC24800.034.164042
CAACGCA26200.032.468515
TCAACGC26250.032.2754634
ATCAACG26400.032.2239533
AACGCAG26850.031.8079686
AGAGTAC29250.029.19808211
ACGCAGA29600.028.8528397
CGCAGAG30200.028.3936318
AGTTACG500.00779888227.5534342
GTAATAC7500.027.094211
CAGAGTA32150.026.56435410
GGTATCA16200.026.1502511
TAATACA7850.025.8861892
TCTGGAG14450.025.34007370
AATACAC8200.025.2013133
CGATTAC700.001293445824.77322845
TCATTTG8850.024.58592836
Produced by FastQC (version 0.11.5)