FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1138423
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTCTTCATTTGCAGAAACA16500.14493733875721063No Hit
GTGTATTACTGTGCGAAGGCGGGGGTCGTTCGAGGTTCGGGGAGTTATTGGTCAAGGAACGCTGGTGCGAATTA15160.1331666700338978No Hit
GTGTTGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCCACTGAACCT13900.12209872780152896No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13720.12051759319690483No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12040.10576033688707977No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11470.10075341063910338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4050.039.9272231
GTATCAA24450.038.9786871
CGCGTAT400.002640618534.38118412
TATCAAC28100.033.8873062
ATCAACG29350.032.4355133
TCAACGC29700.031.941774
CAACGCA29600.031.9335615
AACGCAG30200.031.4129336
AGAGTAC32950.029.00494411
ACGCAGA33600.028.336547
CGCAGAG34400.027.5776358
CAGAGTA35200.027.14616610
TAAACGT4900.025.59251251
AGTACGG33150.025.09878213
GGTATCA16800.024.9848541
CTAAACG4900.024.86389750
GAGTACG34100.024.29872712
CGATTCA7050.024.00282970
ACGGGGG5350.023.78182216
GCGAATT6050.023.7466367
Produced by FastQC (version 0.11.5)