FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059844
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26480.2498480908511064No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAACG200.00666772353.0568659
ACGGATA200.007638619351.26392412
GTATCAA31400.040.7737351
GGTATCA12650.040.5918961
TATCAAC36000.035.3301542
TCAACGC35950.035.072454
ATCAACG36400.035.0193063
CCGAACG400.00241981334.9985549
CAACGCA36400.034.736015
AACGCAG38450.033.1506546
ACGCAGA40500.031.4711727
CGCAGAG41600.030.7182458
AGAGTAC42050.030.47651911
TATCGAC802.782972E-630.15730735
GCAGAGT43050.029.68369
CAGAGTA43250.029.62673410
AGTACGG31600.028.33575213
GAGTACG32500.027.8665412
TACGGGA12200.025.7744615
GTGGTAT4650.025.7663941
Produced by FastQC (version 0.11.5)