FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162002
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT34950.300774008994821No Hit
GTATAGGGGCCTGGCTTGGCCTCTGCTGGTACCAATAAGCATATTGCTTT31780.2734935051746899No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCTCCGAAAAGTGCCACTGCTGTCTG26450.2276243930733338No Hit
CTATTACTGTCAGTCAGCAGACAGCAGTGGCACTTTTCGGAGAGTGTTCG26280.22616140075490404No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25930.2231493577463722No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC25600.22030943148118506No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCTCCGAAAAGTG24920.21445746220746606No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC24630.2119617694289683No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24360.20963819339381518No Hit
GTGTCAGTGTCCCCGGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC23980.20636797527026635No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTCCCCGGGACAGACGG22750.19578279555456873No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21730.18700484164399028No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTCCCCGGGACAGACGGCCAGGATCAC21320.18347644840542443No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG20810.1790874714501352No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20400.17555907821156935No Hit
GGCTTGGCCTCTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGCCGT20330.17495666960986297No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20310.1747845528665183No Hit
CAGTAATAGTCACCCTCGTCCTCTGCCCGGACTCCACTGATGGTCAACGT19910.17134221799962476No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19780.1702234591678844No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC19250.1656623654692505No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19240.16557630709757815No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19200.1652320736108888No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC18870.16239214734570165No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18710.16101521339894423No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18540.15955222108051448No Hit
CCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTCCCCGGGACAGACGGCCAGGATCACCTGCTCTGGAGATG18330.1577449952753954No Hit
TCAGTGGAGTCCGGGCAGAGGACGAGGGTGACTATTACTGTCAGTCAGCA17420.14991368345321265No Hit
GGCCAAGCCAGGCCCCTATACTATTAATATATGAGGACACTGAGAGGCCC17150.14759010741805953No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16960.14595499835628511No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16840.14492229789621705No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16590.1427708386044086No Hit
CCCCTATACTATTAATATATGAGGACACTGAGAGGCCCTCAGGGATCCCT16460.14165207977266822No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16220.1395866788525321No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16150.13898427025082574No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16060.13820974490577467No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACA15980.13752127793239596No Hit
GGACGAGGGTGACTATTACTGTCAGTCAGCAGACAGCAGTGGCACTTTTC15930.1370909860740343No Hit
CTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGCCGTCTGTCCCGGGGA15930.1370909860740343No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC15410.13261595074707272No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15400.1325298923754004No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15240.131152958428643No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14940.12857120727847285No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGT14700.12650580635833675No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14570.12538704752659632No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14350.12349376334980491No Hit
CCCCGGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAG14120.12151442080134114No Hit
GTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACC14110.1214283624296688No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCG13800.11876055290782632No Hit
CACTGACACCGAGGGCGGCTGTGTCAGCTCATAGGAGGCCTCAGAGCCTG13760.11841631942113696No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13430.11557639315594981No Hit
GTAATAGTCACCCTCGTCCTCTGCCCGGACTCCACTGATGGTCAACGTGA13310.11454369269588176No Hit
GCATATTGCTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGCCGTCTG13290.11437157595253708No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13230.11385522572250308No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13160.11325281712079668No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12840.11049894922728189No Hit
CCCTCGTCCTCTGCCCGGACTCCACTGATGGTCAACGTGACTGTTGTCCC12800.11015471574059253No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT12570.10817537319212876No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC12550.10800325644878409No Hit
GACTATTACTGTCAGTCAGCAGACAGCAGTGGCACTTTTCGGAGAGTGTTCGGCGGAGGGACCAAGCTGACCGTC12420.10688449761704368No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG12420.10688449761704368No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12350.10628208901533732No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCTCCGAAAAGTGC12340.106196030643665No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCT12110.10421668809520121No Hit
GTCCCGGGGACACTGACACCGAGGGCGGCTGTGTCAGCTCATAGGAGGCC11930.10266763740509914No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11800.10154887857335873No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11770.10129070345834172No Hit
CTATTAATATATGAGGACACTGAGAGGCCCTCAGGGATCCCTGAGCGATT11680.1005161781132907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG8450.062.12660270
TATACAC6000.048.8122373
TTATACA6300.046.4918442
CTTATAC6600.044.3804861
AAGGGGG7950.043.89841569
GTGGTAT3500.043.746481
GGTATCA11300.041.827631
TACACAT7750.039.0784155
CGTATGC7350.038.41092741
TGGTATC4000.038.2765242
CTCGTAT6700.038.26769639
TCGTATG7300.038.03812440
GTATCAA27400.037.9015851
ACGAGAC7850.036.891122
ATACACA7950.036.8394244
AATCTCG6950.036.5454636
TCTCGTA6850.036.3080138
GCTAATC7100.036.02477333
CCCACGA8050.035.97455619
CGAGACT8300.035.29506723
Produced by FastQC (version 0.11.5)