FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420860
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6060.14399087582569026No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC4350.10335978710259944No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGTT200.00339204662.92564469
CGGGTAA200.007587814751.3451117
GTGGTAT1950.040.373931
GGTATCA6400.039.043681
GTATCAA15050.035.0261231
CGCATTC450.00459438430.7006536
TATCAAC17500.030.4846522
TCAACGC17400.030.452464
ATCAACG17300.030.430883
TTAGCAA450.00483112630.386974
CAACGCA17350.030.3431845
AACGCAG17550.029.8026056
ACGCAGA17750.029.6593917
TGGTATC2600.028.9363692
CGAATGC605.683011E-428.51488516
AGAGTAC18500.028.47390211
AGCCGTT500.00684405928.296362
ACAATCG500.00701835728.1509658
CAGAGTA19050.027.99429510
CGCAGAG19150.027.6695968
Produced by FastQC (version 0.11.5)