FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205895
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATAGTAGCTCACCTCAGACGTTCGGCCAAGGGA43070.35716210781204No Hit
GTATAGTAGCTCACCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT39490.3274746142906306No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38990.32332831631278014No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA34990.29015793248997634No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA33110.2745678520932585No Hit
CTACTATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC28870.2394072452410865No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28300.23468046554633695No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25520.2116270487894883No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24790.20557345374182662No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21830.1810273697129518No Hit
GTGTATTACTGTCAGCAGTATAGTAGCTCACCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA21210.1758859602204172No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACC20710.1717396622425667No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG20370.1689201796176284No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT20190.16742751234560224No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20040.1661836229522471No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19290.15996417598547139No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT19100.1583885827538882No Hit
CCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG18860.15639835972451996No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC18570.15399350689736668No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18480.1532471732613536No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18420.15274961750401153No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18350.15216913578711247No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17540.1454521330629947No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC17140.1421350946807143No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16970.14072535336824515No Hit
GTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGA16890.14006194569178906No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGC16810.13939853801533303No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16350.13558394387571057No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16190.13425712852279842No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT16040.13301323912944327No Hit
GTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCAGATACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAA15200.12604745852665447No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15010.12447186529507129No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14930.12380845761861521No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC14750.12231579034658906No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14730.12214993842747503No Hit
CATCTATGGTGCAACCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA14390.1193304558025367No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14350.11899875196430866No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA14100.11692560297538342No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATAGTAGCTCA13880.11510123186512923No Hit
GCAGATACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAACCAACAGGGCCACTGGCATCCCAGACAGGTT13800.11443782418867315No Hit
GTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCAGATACCTGGC13560.11244760115930491No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC13370.11087200792772173No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13360.11078908196816473No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12850.10655985803075725No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC12800.10614522823297219No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12740.10564767247563014No Hit
CGTCTGAGGTGAGCTACTATACTGCTGACAGTAATACACTGCAAAATCTT12540.10398915328448996No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12390.1027452638911348No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12270.10175015237645069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9900.043.8375661
GTATCAA25800.041.5898931
TATACAC2950.037.0670363
TATCAAC29250.036.799732
CTTATAC2900.036.529321
TCAACGC30900.035.27854
CAACGCA31550.034.44485
ATCAACG31550.034.3336373
TCGTATG3050.033.9417440
AACGCAG32150.033.694286
CGTATGC3150.032.89866341
ACGCAGA33250.032.5795827
CGAGACG3050.032.50275423
TTATACA3400.032.1611022
AGAGTAC34800.031.22538211
ACGAGAC3300.031.0737222
CGCAGAG35200.030.7747468
GCCGTCT3650.030.44944846
GCAGAGT36150.029.9660079
CACGAGA3700.028.6358421
Produced by FastQC (version 0.11.5)