FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2155968
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA98810.45830921423694604No Hit
GGCCTACAGTGTACGGCCAGTGTGTACCTTGCATGCAGTAATAAACCCCA80980.37560854335500343No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT78670.36489409861370853No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC68640.3183720723127616No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA66910.31034783447620745No Hit
GTGTACGGCCAGTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTC57200.26531006026063464No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56450.26183134443553896No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTT49900.2314505595630362No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC47960.22245228129545522No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG46070.21368591741621396No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT44500.2064038056223469No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG43380.20120892332353726No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41430.19216426217828836No Hit
GGTACACACTGGCCGTACACTGTAGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT40680.18868554635319262No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG38090.17667238103719535No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGATACACTGCCG37070.17194132751506516No Hit
GGCCGTACACTGTAGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC36970.17147749873838572No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36950.17138473298304982No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA36770.17054984118502686No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG36680.1701323952860154No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC36000.16697835960459523No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34700.16094858550776264No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34460.159835396443732No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34200.15862944162436549No Hit
GTGTATCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAG34030.15784093270401045No Hit
GTACACACTGGCCGTACACTGTAGGCCAGGGGACCAAGCTGGAGATCAAA33670.1561711491079645No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33130.15366647371389555No Hit
CAATAATATACAGCCGTGTCGTCGGCTCTCAGTCTGTTCAACTGAAGATC32910.15264605040520082No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCTGTGGGGATGTTGTCATGACTCAGTCTCCACTCTCC32810.15218222162852138No Hit
GTATCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGA32750.1519039243625137No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATGACAACATCC32340.15000222637812807No Hit
GCTAATGCTCTGGGTCCCAGGATCCTGTGGGGATGTTGTCATGACTCAGT32050.1486571229257577No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31840.1476830824947309No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTGACCGGGAC31650.14680180781903998No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31430.14578138451034522No Hit
GTCTGGGACCCCAGAGTCCCGGTCAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA30960.14360138925995192No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACA30530.14160692552023035No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30310.1405865022115356No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30200.14007629055718823No Hit
AGGTACACACTGGCCGTACACTGTAGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT30100.1396124617805088No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCTGTGGGGATGTTGTCATGA29840.1384065069611423No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATC29390.13631927746608483No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG29080.1348814082583786No Hit
GTCCCAGACAGATTCAGCGGCAGTGTATCAGGCACTGATTTCACACTGAA28390.1316809896992905No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27910.12945461157122926No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCTGTGGGGATGTTGTCATGACTCAGTCTCCACT27820.12903716567221776No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27790.12889801703921394No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCTACAGTG27760.12875886840621012No Hit
CTCGTACACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCC26690.1237959004957402No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25970.1204563333036483No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25770.11952867575028943No Hit
CCATATACATGGGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCTCAGTTATTTGGACCGGTGGTA25600.11874016682993438No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25120.11651378870187314No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24430.11331337014278504No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24330.11284954136610562No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC23960.11113337489239172No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA23940.11104060913705585No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA23930.1109942262593879No Hit
ATAATATACAGCCGTGTCGTCGGCTCTCAGTCTGTTCAACTGAAGATCCA23620.10955635705168167No Hit
GTGTACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGA23280.1079793392109716No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC22640.10501083504022322No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT22250.10320190281117345No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC22160.10278445691216195No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22130.10264530827915815No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21890.10153211921512749No Hit
GGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGTATCAGGCACTGATT21790.10106829043844806No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC21770.10097552468311217No Hit
CTCCTGTGCAGCCTCTGGATTCACCGTCACCACCATATACATGGGCTGGGTCCGCCAGGCTCCAGGGAAGGGG21650.10041893015109687No Hit
GGTCTAGTCAAAGCCTCGTACACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTC21580.10009425000742127No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)