FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2155968
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA89650.41582249829311013No Hit
GGCCTACAGTGTACGGCCAGTGTGTACCTTGCATGCAGTAATAAACCCCA79200.36735239113010953No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT75600.35065455516965No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC71370.33103459791611006No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA54490.2527403004126221No Hit
GTGTACGGCCAGTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTC51490.23882543711223914No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG49730.23066205064268117No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49170.22806460949327634No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTT46400.2152165523792561No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45150.2094186926707632No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT44850.2080272063407249No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41900.1943442574286817No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39610.18372257844272272No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA39040.18107875441564994No Hit
GGTACACACTGGCCGTACACTGTAGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT38270.17750727283521833No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38240.1773681242022145No Hit
GGCCGTACACTGTAGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC38100.1767187639148633No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGATACACTGCCG36460.16911197197732064No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC34780.16131964852910619No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG34730.16108773414076646No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34600.16048475673108323No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33720.1564030634963042No Hit
GTGTATCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAG33160.1538056223468994No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32570.15106903256449075No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATGACAACATCC31710.14708010508504765No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31490.1460596817763529No Hit
GTATCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGA31470.145966916021017No Hit
GTCTGGGACCCCAGAGTCCCGGTCAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA31350.1454103214890017No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30890.1432767091162763No Hit
GTACACACTGGCCGTACACTGTAGGCCAGGGGACCAAGCTGGAGATCAAA30520.1415605426425624No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA30520.1415605426425624No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACA30390.14095756523287917No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29760.13803544393979872No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29670.13761799804078725No Hit
GCTAATGCTCTGGGTCCCAGGATCCTGTGGGGATGTTGTCATGACTCAGT29340.1360873630777451No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCTGTGGGGATGTTGTCATGACTCAGTCTCCACTCTCC29330.13604098020007718No Hit
CAATAATATACAGCCGTGTCGTCGGCTCTCAGTCTGTTCAACTGAAGATC29060.13478864250304273No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28940.1342320479710274No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28560.13246949861964558No Hit
GTCCCAGACAGATTCAGCGGCAGTGTATCAGGCACTGATTTCACACTGAA28110.1303822691245881No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTGACCGGGAC27710.1285269540178704No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA27430.12722823344316797No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATC27110.1257439813577938No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG27060.12551206696945408No Hit
AGGTACACACTGGCCGTACACTGTAGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT27010.12528015258111438No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCTGTGGGGATGTTGTCATGACTCAGTCTCCACT26920.12486270668210289No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCTGTGGGGATGTTGTCATGA26770.12416696351708376No Hit
CTCGTACACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCC26630.12351760322973253No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCTACAGTG26430.12258994567637367No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26330.12212611689969424No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25750.11943590999495354No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25700.11920399560661384No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24330.11284954136610562No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC24290.11266400985543384No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23970.11117975777005966No Hit
CCATATACATGGGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCTCAGTTATTTGGACCGGTGGTA23660.10974188856235344No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA22990.10663423575860123No Hit
GCCTGATACACTGCCGCTGAATCTGTCTGGGACCCCAGAGTCCCGGTCAG21980.10194956511413898No Hit
GTGTACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGA21880.10148573633745955No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21710.10069722741710453No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21590.1001406328850892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5950.044.2209631
GGTATCA17600.041.1602131
GTATCAA42600.039.5450131
TGGTATC6650.039.0205652
CATCTGA37150.037.62473370
TATCAAC47500.035.221192
TACGTTT400.002419137435.00221352
ATCAACG51050.032.760523
TCAACGC51650.032.317624
CAACGCA52350.032.017385
AACGCAG52650.031.770116
AGAGTAC54850.030.55948311
CAGAGTA55850.030.07064610
GACCGGA900.00513799230.0109270
GCAGAGT56150.029.7269179
CGCAGAG56850.029.421618
ATCAGTG34900.029.21550670
AGTACGG54200.026.95849613
GAGTACG54700.026.77386512
AAACGGT907.0494025E-626.7643136
Produced by FastQC (version 0.11.5)