FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1820261
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT70680.38829596415019607No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55960.30742844020720106No Hit
CAGTAATAGACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA53950.29638606771226766No Hit
CTATTACTGCATGCAAACTCTACAAACTCCTCGGACGTTCGGCCAAGGGA53940.2963311305356759No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG51740.28424495168550007No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGG49890.27408157401603395No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49700.2730377676607915No Hit
GTAATAGACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA49100.26974153706528897No Hit
GGTCTAGTCAGAGCCTCCAGCATTCTAATGGAAACAACTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTC48390.2658409975272777No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43520.2390865925271156No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41560.22831890591514076No Hit
GTCTATTACTGCATGCAAACTCTACAAACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA40900.22469305226008798No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC40140.2205178268391181No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38380.21084888375897745No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38150.20958532869736812No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA38060.20909089410804274No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36490.2004657573831445No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG35330.19409304489850632No Hit
GTCAGGGACCCCGGCGGCCCGCTTAGAACCCAAACAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA35220.19348873595599753No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC35120.19293936419008043No Hit
CTCTACAAACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA34990.19222518089438823No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT34410.18903882465206912No Hit
GTCTCCACTCTCCCTGCCCGTCACCGCTGGAGAGCCGGCCTCCCTCACCT34380.188874013122294No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA33200.18239142628447239No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33010.18134761992922993No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA32490.17849088674646107No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32140.1765680855657513No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32020.17590883944665078No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31860.17502984462118343No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31730.17431566132549123No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGATATTGTGATGA31350.1722280486150063No Hit
GCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCG31250.17167867684908925No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG31110.1709095563768053No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31080.1707447448470302No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGATATTGTGATGACTCAGTCTCCACT30140.16558065024740956No Hit
GTAATATATAGCCGTGTCGTCAGCTCTCAGCCTGCTCACTTGCAGATACA29690.16310847730078268No Hit
GACTAGACCTGCAGGTGAGGGAGGCCGGCTCTCCAGCGGTGACGGGCAGG29530.16222948247531535No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC28630.15728513658206159No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG28590.15706538787569474No Hit
ATATATTACTGTGCGAAAGATCATGACATTCGGGGAGTTGTTTACGACTT28420.1561314558736357No Hit
GTTTGGGTTCTAAGCGGGCCGCCGGGGTCCCTGACAGGTTCAGTGGCAGT28380.15591170716726888No Hit
CTACAAACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG28020.15393396880996738No Hit
CTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC27460.15085748692083167No Hit
GTTTGTAGAGTTTGCATGCAGTAATAGACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT27440.15074761256764826No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26410.14508908337870227No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTGTTTGGGTTCTAAGCGGGCC26330.1446495859659686No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26320.14459464878937692No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG26130.14355084243413443No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTCTATTACTGCATGCAAACTCTACAAACT25820.14184778995979147No Hit
GATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAGTCCAAAT25740.1414082925470578No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25430.13970524007271484No Hit
TCCGAGGAGTTTGTAGAGTTTGCATGCAGTAATAGACTCCAACATCCTCA24940.13701331841972111No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24710.13574976335811184No Hit
GCCCTGGCTTCTGCAGGTACCAGTCCAAATAGTTGTTTCCATTAGAATGC24680.13558495182833671No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT24190.13289303017534299No Hit
CTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTGTTTGGGTTCTAAGCGGGCCGC24180.1328380929987513No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG23870.1311350405244083No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23640.12987148546279903No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23610.12970667393302387No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23330.12816843298845607No Hit
GTCCAAATAGTTGTTTCCATTAGAATGCTGGAGGCTCTGACTAGACCTGC23220.12756412404594725No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23200.12745424969276384No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC22570.1239932075674862No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21830.11992785649969978No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTGTTTGGGTTCTAAGCGGGCCGCCGGGGTCCCTGA20510.11267614918959423No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA20420.11218171460026886No Hit
GAGTTTGTAGAGTTTGCATGCAGTAATAGACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT20180.11086322236206786No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC20170.11080828518547614No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20130.11058853647910932No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT19700.10822623788566586No Hit
CTCCCTGCCCGTCACCGCTGGAGAGCCGGCCTCCCTCACCTGCAGGTCTA19620.10778674047293219No Hit
GTCTCCACAGCTCCTGATCTGTTTGGGTTCTAAGCGGGCCGCCGGGGTCC19570.10751205458997364No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19500.10712749435383168No Hit
GAGTGGAGGCTGAGGATGTTGGAGTCTATTACTGCATGCAAACTCTACAAACTCCTCGGACGTTCGGCCAAGGGA19500.10712749435383168No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19430.10674293411768972No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT19310.10608368799858922No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGATATTGTGATGACTCAGTCTC19130.10509481881993846No Hit
CTCCACAGCTCCTGATCTGTTTGGGTTCTAAGCGGGCCGCCGGGGTCCCT19080.10482013293697992No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC19080.10482013293697992No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19010.10443557270083796No Hit
GTGGAGACTGCCCTGGCTTCTGCAGGTACCAGTCCAAATAGTTGTTTCCA18810.10333682916900377No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC18780.10317201763922867No Hit
GCTCCTGATCTGTTTGGGTTCTAAGCGGGCCGCCGGGGTCCCTGACAGGT18740.10295226893286183No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18570.10201833693080278No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18530.10179858822443595No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTGTTTGGGTTCTAAGCGGGCCGCCGGGGTCCCTGACAGGTT18420.10119427928192715No Hit
GTGGAGGCTGAGGATGTTGGAGTCTATTACTGCATGCAAACTCTACAAAC18380.10097453057556031No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18310.10058997033941836No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTCG350.005142198456.65749770
GGTATCA14950.045.1034161
GTGGTAT4500.040.1096421
TGGTATC4850.039.315172
GTATCAA36100.039.2438471
TATACAC2950.035.781093
TATCAAC41600.034.4589962
CTTATAC2800.034.055361
CGCTAAT2900.033.00526432
TCAACGC44750.032.3386344
GCTAATC3100.032.0015533
ATCAACG45050.031.9712373
TCGCTAA3000.031.8804731
CAACGCA45600.031.7367045
AACGCAG46400.031.2629096
ATGTGTA2300.031.08975411
CGGACTG450.00492887830.2681035
GGCCCGG12050.030.17031370
TCGTATG3300.029.25634640
AGAGTAC49650.029.1471111
Produced by FastQC (version 0.11.5)