FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1426643
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGCAC32240.22598505722875312No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30330.2125969846696055No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGCACGTCACTGAGG30250.2120362277037773No Hit
GTAATATGTAGCCATGTCCGCGGCGGTCACAGAGCTCAACTTCAGGGAGAACTGGTTCATGGACGTGTCTACTGA27920.1957041810740318No Hit
GTAATACTCGGCCTCGTCCCCAGTCTGGAGTCCGGCGATGCCCAGGGTGG27000.18925547596700787No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26950.18890500286336526No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26050.18259648699779835No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT25560.17916185058210077No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC24640.17271314547507682No Hit
GTGTCTGGTGGGTCCTTCAGTAGTTATTATTGGAGCTGGATCCGCCAGTA24120.1690682251971937No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22870.1603063976061285No Hit
CAGTAATATGTAGCCATGTCCGCGGCGGTCACAGAGCTCAACTTCAGGGAGAACTGGTTCATGGACGTGTCTA22650.15876431595010104No Hit
GAGTATTACTGCGTAACCTGGGATAGCATCCTCAGTGACGTGCTCTTCGG22310.1563810988453313No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC22220.15575024725877462No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22030.1544184494649327No Hit
GGACAGAGGGTCGCCATCTCCTGCTCTGGAAGCAGATCCAACATTGGGAA22000.15420816560274717No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21540.15098381304923517No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21130.14810993359936578No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20690.14502577028731084No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20500.14369397249346894No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC20370.14278274242399816No Hit
ACATATTACTGTGCGCGGGGTCAATCGGCTCACACCACTGACTGCACTACCACAACCTGCTTTAGTCAGGGACGG20180.14145094463015626No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19700.13808640283518722No Hit
GTATTACTGCGTAACCTGGGATAGCATCCTCAGTGACGTGCTCTTCGGCG19660.13780602435227313No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19100.13388072559147593No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG19090.1338106309707474No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18920.13261902241836254No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18560.13009561607213577No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC18430.129184386002665No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17530.12287587013709808No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17420.12210482930908433No Hit
CAGTAATACTCGGCCTCGTCCCCAGTCTGGAGTCCGGCGATGCCCAGGGT17020.11930104447994347No Hit
ACCTGGGATAGCATCCTCAGTGACGTGCTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG16810.11782905744464453No Hit
ATCCCAGGTTACGCAGTAATACTCGGCCTCGTCCCCAGTCTGGAGTCCGG16440.11523555647768922No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG16420.1150953672362322No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16330.1144645156496755No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16180.11341309633874767No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCGCCATCTCCTGCTCTGG15930.11166073082053464No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15820.11088968999252091No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15760.11046912226814977No Hit
CTCCTGCTCTGGAAGCAGATCCAACATTGGGAACAATTTTGTATCTTGGTACCAGCAGTTTCCAGGAACAGCCCC15680.1099083653023216No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15600.10934760833649343No Hit
GTCTGGAGTCCGGCGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGCC15500.10864666212920822No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15400.10794571592192301No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA15190.10647372888662404No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14900.10444098488549693No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14870.10423070102331138No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC14830.10395032254039728No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14500.10163720005635607No Hit
GTCCCTCACCTGCGCTGTGTCTGGTGGGTCCTTCAGTAGTTATTATTGGA14430.10114653771125642No Hit
GCCCAGGGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGGCTGGAA14360.10065587536615676No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14310.10030540226251418No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)