FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1426643
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30810.2159615264645745No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28920.202713643146884No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGCAC26330.18455913637819693No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT26200.18364790630872616No Hit
GTAATATGTAGCCATGTCCGCGGCGGTCACAGAGCTCAACTTCAGGGAGAACTGGTTCATGGACGTGTCTACTGA24870.1743253217518328No Hit
GTATTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCGC24570.17222248312997715No Hit
GTGTCTGGTGGGTCCTTCAGTAGTTATTATTGGAGCTGGATCCGCCAGTA24540.1720121992677916No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23680.16598406188513876No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23410.1640915071254687No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGCACGTCACTGAGG23280.1631802770559979No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23170.16240923622798414No Hit
GTCCTGGGCCCAGTCTGTATTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCGCCATCTCCTG21890.1534371247747334No Hit
GGACAGAGGGTCGCCATCTCCTGCTCTGGAAGCAGATCCAACATTGGGAA21850.15315674629181933No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21470.15049315070413552No Hit
CAGTAATATGTAGCCATGTCCGCGGCGGTCACAGAGCTCAACTTCAGGGAGAACTGGTTCATGGACGTGTCTA21300.14930154215175065No Hit
GAGTATTACTGCGTAACCTGGGATAGCATCCTCAGTGACGTGCTCTTCGG21240.14888097442737952No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20090.14082009304359955No Hit
GTAATACTCGGCCTCGTCCCCAGTCTGGAGTCCGGCGATGCCCAGGGTGG19310.1353527126267749No Hit
ACATATTACTGTGCGCGGGGTCAATCGGCTCACACCACTGACTGCACTACCACAACCTGCTTTAGTCAGGGACGG19300.13528261800604635No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19170.13437138793657558No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19150.13423119869511854No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19040.1334601578671048No Hit
GTATTACTGCGTAACCTGGGATAGCATCCTCAGTGACGTGCTCTTCGGCG18490.12960495372703612No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18470.1294647644855791No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18410.12904419676120796No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC18200.12757220972590902No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC17660.12378710020656886No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT17500.1226655862749125No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17460.12238520779199842No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17390.12189454544689877No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG17380.12182445082617026No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17380.12182445082617026No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17280.12112350461888502No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG16880.11831971978974416No Hit
ACCTGGGATAGCATCCTCAGTGACGTGCTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG16810.11782905744464453No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16420.1150953672362322No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16380.11481498875331811No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16230.11376356944239029No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16210.11362338020093324No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16100.11285233937291951No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15680.1099083653023216No Hit
CTCCTGCTCTGGAAGCAGATCCAACATTGGGAACAATTTTGTATCTTGGTACCAGCAGTTTCCAGGAACAGCCCC15490.10857656750847969No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA15370.10773543205973744No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCGCCATCTCCTGCTCTGG15060.10556249881715327No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14910.10451107950622544No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14820.10388022791966876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)