FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1286724
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26540.20626023918105205No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGCAC25160.19553532847759117No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGCACGTCACTGAGG24590.19110547405659645No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23500.1826343489357469No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21660.16833446799779905No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21180.16460406427485616No Hit
GTAATACTCGGCCTCGTCCCCAGTCTGGAGTCCGGCGATGCCCAGGGTGG20630.1603296433423174No Hit
GTAATATGTAGCCATGTCCGCGGCGGTCACAGAGCTCAACTTCAGGGAGAACTGGTTCATGGACGTGTCTACTGA20550.15970790938849358No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19970.15520033822327087No Hit
GAGTATTACTGCGTAACCTGGGATAGCATCCTCAGTGACGTGCTCTTCGG18920.14704008007933325No Hit
CAGTAATATGTAGCCATGTCCGCGGCGGTCACAGAGCTCAACTTCAGGGAGAACTGGTTCATGGACGTGTCTA18720.1454857451947737No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC17760.13802493774888788No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17660.1372477703066081No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17360.1349162679797688No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17310.1345276842586289No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16880.13118586425682585No Hit
GTGTCTGGTGGGTCCTTCAGTAGTTATTATTGGAGCTGGATCCGCCAGTA16810.13064184704723003No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16800.13056413030300204No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16760.13025326332609014No Hit
GTATTACTGCGTAACCTGGGATAGCATCCTCAGTGACGTGCTCTTCGGCG16350.12706687681274306No Hit
GGACAGAGGGTCGCCATCTCCTGCTCTGGAAGCAGATCCAACATTGGGAA15840.12310332285711621No Hit
ACATATTACTGTGCGCGGGGTCAATCGGCTCACACCACTGACTGCACTACCACAACCTGCTTTAGTCAGGGACGG15370.11945063587840128No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG15180.1179740177380697No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG15070.11711913355156195No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14890.11572023215545835No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14870.1155647986670024No Hit
ACCTGGGATAGCATCCTCAGTGACGTGCTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG14580.11331101308439107No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14450.11230069540942736No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14280.11097951075755173No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14150.10996919308258803No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC14060.10926974238453624No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13960.10849257494225646No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13900.10802627447688859No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13900.10802627447688859No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13740.10678280656924094No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13730.10670508982501298No Hit
CAGTAATACTCGGCCTCGTCCCCAGTCTGGAGTCCGGCGATGCCCAGGGT13340.10367413680012186No Hit
GTCCTGGGGCCGCAGACACTGAGGGCGGCTGCGTCAATACAGACTGGGCC13120.10196436842710636No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13080.10165350145019444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)