FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205291
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTACACAGCCGTGTCCGCGGCGGTCACAGAGGTCAAGTTCAGGGAGA16080.1334117652915354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGAT5900.039.0068770
GTATCAA26700.037.9741481
TATCAAC30050.034.537092
ATCAACG30900.033.587043
TCAACGC31550.032.7861444
CAACGCA32150.032.494955
AACGCAG32450.032.088636
GGTATCA17350.030.9031281
ACGCAGA36200.028.7645327
AGAGTAC36250.028.72485711
GTGGTAT3650.028.249171
CCGAAAC805.5574386E-528.02246968
CGCAGAG37400.027.749728
CAGAGTA38050.027.36599310
GCAGAGT38950.026.7336629
AGTACGG31100.025.85703513
CGAACTA809.7531425E-525.79984929
GAGTACG31450.025.46000512
ACGTGTC4900.025.25938463
CGTGTCT5450.023.39509264
Produced by FastQC (version 0.11.5)