FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205291
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTACACAGCCGTGTCCGCGGCGGTCACAGAGGTCAAGTTCAGGGAGA14660.12163037805807891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28800.040.4595951
TATCAAC31000.037.0969472
ATCAACG32300.035.4828723
TCAACGC32400.035.3718874
GTGGTAT5250.034.7001081
CAACGCA33500.034.4152765
AACGCAG34100.033.8097276
AGAGTAC36550.031.44953311
TATGGAT5800.030.80944670
ACGCAGA37500.030.6528137
CAGAGTA37650.030.34715810
CGCAGAG38700.029.5237878
GCAGAGT40000.028.5642629
AGTACGG34100.028.07531513
GAGTACG34400.027.5312212
GGTATCA16900.026.6439691
ACGTGTC4500.025.1505663
ACGACGT700.001374505924.51629817
TACGGGG19750.023.80577715
GTACGGG40000.023.67783214
Produced by FastQC (version 0.11.5)