FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693939
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT17970.25895647888359064No Hit
GTGTATTACTGTCAGCACTATGATACCTCACCTATGTACACTTTTGGCCA15990.23042371159424677No Hit
GTAATACACTGCACAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA14640.21096955207878504No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14580.21010492276698672No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCTGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA14050.20246736384610173No Hit
GTATTACTGTCAGCACTATGATACCTCACCTATGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAA12930.1863276166925335No Hit
CAGTAATACACTGCACAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12120.17465512098325645No Hit
GTAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAACGACTACTTACACTCTGA11690.16845861091536865No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11110.1601005275679851No Hit
CTATGATACCTCACCTATGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT10870.1566420103207919No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10740.15476864681189556No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10540.15188654910590124No Hit
CCTCATCTACAGTGCATCCATCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT9430.13589090683763272No Hit
CTCCTGCAGGGCCAGTCAGAGTGTAAGTAGTCGTTACTTAGCCTGGTACC9410.13560269706703326No Hit
CATCTACAGTGCATCCATCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA9140.13171186516394093No Hit
GTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACC9090.13099134073744234No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9010.12983850165504462No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGAT8400.1210481036517619No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8120.11701316686336984No Hit
CTGTAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAACGACTACTTACACTCTGA7980.1149956984691738No Hit
GAGTGTAAGTAGTCGTTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG7820.11269002030437833No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7810.11254591541907863No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGC7750.11168128610728031No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCT7740.11153718122198061No Hit
GTATCATAGTGCTGACAGTAATACACTGCACAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC7640.11009613236898344No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7530.10851097863068657No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA7480.10779045420418797No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7480.10779045420418797No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7390.10649351023649054No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7230.10418783207169506No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7050.10159394413630017No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCTGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC6960.10029700016860271No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6940.10000879039800327No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)