FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564114
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7940.1407516920338797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13900.038.978691
TCAACGC15950.034.1414644
CAACGCA16000.034.034775
ATCAACG16000.034.0317543
AACGCAG16650.032.9117856
GGTATCA6900.032.800451
TATCAAC16600.032.601172
AGAGTAC18050.030.17199911
ACGCAGA18300.030.1314817
CGATAAG704.1820586E-430.0492369
CAGAGTA18450.029.51524710
GTGGTAT2450.029.3926141
TAAACGG704.044247E-529.3561345
CGCAGAG19000.028.8411168
GCAGAGT19100.028.5108019
AGTACGG16450.028.1094313
GAGTACG16700.027.8912612
TCGTTGG907.161205E-626.70425628
CCGTTAA801.0033791E-425.68889616
ATAAACG851.5153176E-424.1756424
Produced by FastQC (version 0.11.5)