FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146719
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA43470.3790815361043115No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42210.36809366549259237No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34000.2964980958717872No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29870.2604822977555966No Hit
GTCTGAGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT26350.22978602430063513No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC25690.2240304730278298No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGAGACAGAGTTCACT23600.20580456066394645No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23370.2037988382506961No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCAATCTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT23240.20266516906059812No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGTGGGAGGCCAGTTATTAAA23200.20231634777133717No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23190.20222914244902193No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC22820.1990025455233584No Hit
GTCTCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT22680.19778167101094515No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21870.19071803990341138No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGAGACAGAGTTCA21830.19036921861415046No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCAATCTAGCCTGGTA21620.1885379068455306No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21610.18845070152321539No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAG21540.18784026426700876No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT20780.18121265977105117No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGTGGGAGG20660.18016619590326838No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20150.17571872446519157No Hit
GTTTAATAACTGGCCTCCCACGTGGACGTTCGGCCAAGGGACCAAGGTGG19880.17336418076268031No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19620.1710968423824843No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19190.16734701352292933No Hit
GTTATTAAACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT18900.16481805917578762No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTTTAATAACTGG18390.1603705877377108No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18330.15984735580381942No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18020.15714399081204725No Hit
GTTTATTACTGTCAGCAGTTTAATAACTGGCCTCCCACGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA17880.155923116299634No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17870.15583591097731878No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCTCAGACCCACTGCCA17460.15226049276239428No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17360.15138843953924197No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG17320.15103961824998105No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17290.15077800228303534No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG16080.14022615828289234No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16020.13970292634900092No Hit
GTGTTAGTAGCAATCTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCA15760.1374355879688049No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14870.1296743142827493No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14570.12705815461329237No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14380.12540125348930295No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTC14160.12348273639836786No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14010.12217465656363939No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14000.12208745124132417No Hit
GAGTGAACTCTGTCTCAGACCCACTGCCACTGAACCTGGCTGGGATACCA13820.12051775543964999No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13260.11563425738999701No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13020.11354132965443148No Hit
GGTTTCTGCTGGTACCAGGCTAGATTGCTACTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC12850.11205883917507252No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT12820.11179722320812684No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTT12800.11162281256349638No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12780.11144840191886592No Hit
GTCAGAGTGTTAGTAGCAATCTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG12720.11092516998497454No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC12660.11040193805108313No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12640.11022752740645267No Hit
CAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCTCAGACCCACTGCCA12570.10961709015024604No Hit
TCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGAGACAGAGTTCACT12230.10665210919152818No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12100.10551844000143017No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACACAGACAGGGTGGCTGG12050.10508241338985401No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTC12050.10508241338985401No Hit
GAGTGTTAGTAGCAATCTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATC12040.10499520806753879No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11990.10455918145596262No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11870.10351271758817983No Hit
GTTCAGTGGCAGTGGGTCTGAGACAGAGTTCACTCTCACCATCAGCAGCC11720.10220463775345136No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGTGGGAGGCCAGTTATTAAACTGCTGACAGTAATAAACTGCAAAATCTT11710.10211743243113613No Hit
CCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCC11570.10089655791872289No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC11480.10011171001788582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG200.007615843351.3029331
GTATCAA27400.041.6914331
GGTATCA10350.040.64581
TATCAAC31300.036.3857962
CTCGTAT1102.7284841E-1134.50136639
ATCAACG33200.034.403493
TCAACGC33600.033.9939234
CAACGCA34050.033.64515
AACGCAG34600.033.110286
TATACAC1700.032.1858633
CTTATAC1600.032.064331
CGCAGAG36950.031.004488
AGAGTAC37000.030.68530711
GTTATAC450.00482617730.397763
GCAGAGT38300.029.9116349
CGTATGC1301.9281288E-1029.25523841
TCGTATG1301.9463187E-1029.22431840
TCGACGT1650.029.07435829
ACGCAGA41450.027.6384937
CTCGACG1750.027.40458528
Produced by FastQC (version 0.11.5)