FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956239
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC9610.10049788808028119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27900.043.045031
GGTATCA11250.038.7356071
TCAACGC34450.035.5580674
CAACGCA34850.035.1517755
ATCAACG34850.035.149943
AACGCAG35100.034.9991726
TATCAAC34900.034.9029462
ACGCAGA37100.033.019937
CGCAGAG38750.031.613928
AGAGTAC40000.030.62598811
CGTATGA450.004746813330.50047714
CAGAGTA40250.030.3505110
CTCGTAT1600.030.27402539
AATCTCG1650.029.29022636
GCAGAGT42200.028.9480559
AGTACGG35200.027.4894213
GAGTACG35600.027.27693612
TCGTATG1800.026.93012440
GTGGTAT4300.025.5352821
CGTATGC1951.8189894E-1224.88483641
Produced by FastQC (version 0.11.5)