FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956239
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14870.15550505679019574No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG350.00625813853.91038570
GTATCAA28300.040.6102941
GGTATCA12950.034.4390641
TCAACGC34150.033.6096274
CAACGCA34450.033.31525
ATCAACG34550.033.2205163
AACGCAG34850.032.932826
TATCAAC36250.031.579632
ACGCAGA36450.031.4872077
CGCAGAG38000.030.4717398
AGAGTAC38750.029.79511511
CAGAGTA38750.029.79355610
GCAGAGT39250.029.326749
GTGGTAT5000.028.8175531
CGATTGT1554.4309763E-628.40439670
ACGGGTA3050.028.09075216
AGTACGG37500.026.58573213
GAGTACG38250.026.06444212
CGCCGTA1459.182211E-526.02570370
TATCGAT809.22644E-526.0105542
Produced by FastQC (version 0.11.5)