FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990716
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTCTTCATTTGCAGAAACA30690.3097759600127585No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24940.25173712749163235No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24260.2448734046891339No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23660.23881717868692948No Hit
GTGTTGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCCACTGAACCT23250.23467875758542306No Hit
GTGTATTACTGTGCGAAGGCGGGGGTCGTTCGAGGTTCGGGGAGTTATTGGTCAAGGAACGCTGGTGCGAATTA21100.21297728107752373No Hit
CTCCAGCCCCGTCCCTGGAGCCTGGCGGACCCAGCCCATCCAATAGTTTC20950.2114632245769726No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20070.2025807597737394No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18600.18774300606833846No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18360.18532051566745666No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18250.1842102075670525No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17920.18087928326584007No Hit
CCATCAACACTCTGCAACCTGGAGATTTTGCAACTTACTACTGTCAACAG17780.17946616386532568No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCCTGGCCGAACGTC16540.1669499634607698No Hit
GTAGGAGACAGAGTCACAATCACTTGCCGGGCAAGTCAGGGCATTAGCAACTATTTAAATTGGTATCAGCAGAAA15870.16018717775830815No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15510.15655344215698544No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15490.15635156795691196No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT15370.15514032275647108No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACAATCACTTGCCGGGCAAGTCAGGGCATTAGCAA15280.15423188885614042No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15120.15261689525555255No Hit
GAGTTCACTCTCACCATCAACACTCTGCAACCTGGAGATTTTGCAACTTACTACTGTCAACAGAGTTTCAGTGGC15110.1525159581555158No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15040.15180939845525862No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14550.14686348055345833No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14400.14534942405290718No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT14310.14444099015257653No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC14100.14232131105180496No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14010.1414128771514743No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14000.14131194005143755No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13720.1384857012504088No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCCTGGCCGAACGTCCAAAGGCCA13690.13818288995029857No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13670.1379810157502251No Hit
GATCTATGCAGCATCCACTTTGCAAAGTGAGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCA13650.1377791415501516No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT13310.1343472801489024No Hit
GGGCTGGAGTGGGTGGCCAACATAAAACAAGATGGAAGTGAGAAACACTA13170.13293416074838804No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCTCA13060.13182385264798388No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTCTTCATTTGCAGAAA12960.13081448164761647No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12890.1301079219473593No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12870.1299060477472858No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12590.12707980894625703No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12510.12627231214596313No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12390.12506106694552221No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12240.1235470104449711No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC12090.12203295394441999No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT12030.12142733134419956No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT11970.1208217087439791No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11970.1208217087439791No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCAGTGAGC11720.11829828124306058No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCTCACTTTGCAAAGT11650.11759172154280338No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11640.11749078444276664No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11330.11436173434162768No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11310.11415986014155419No Hit
CTCCTGTGCAGCCTCTGGATTCACGTTTAGAAACTATTGGATGGGCTGGGTCCGCCAGGCTCCAGGGACGGGG11280.11385704884144397No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11250.11355423754133373No Hit
CTATGTGGACTCTGTGGAGGGCCGATTCACCATCTCCAGAGACAACGCCA10680.10780082283923949No Hit
ACTTACTACTGTCAACAGAGTTTCAGTGGCCTTTGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAACGAA10660.10759894863916601No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10480.10578208083850467No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10400.10497458403821076No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10400.10497458403821076No Hit
GTAGTAAGTTGCAAAATCTCCAGGTTGCAGAGTGTTGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCCA10400.10497458403821076No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10370.10467177273810052No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10310.10406615013788006No Hit
CTCCTGATCTATGCAGCATCCACTTTGCAAAGTGAGGTCCCATCAAGGTT10140.10235021943725549No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10120.102148345237182No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10060.10154272263696155No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG9990.10083616293670436No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCAGTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAG9980.10073522583666761No Hit
GTATTACTGTGCGAAGGCGGGGGTCGTTCGAGGTTCGGGGAGTTATTGGTCAAGGAACGCTGGTGCGAATTACTA9910.10002866613641043No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG150.00204988571.45994652
TAAGAGG350.00396634560.49285570
AAGGGGG3350.051.94854469
CGTATGC2800.051.6196841
TATACAC2850.051.5198673
GTGGTAT2350.051.1315841
TCGTATG2900.049.68966340
ATCTCGT2900.049.29820337
CTCGTAT2950.048.70842739
TTATACA3050.048.1463742
CTTATAC3000.047.841171
CCGTCTT2950.046.34205647
ATGCCGT3050.045.51101744
CATCTCG3100.044.89249836
TGCCGTC3150.044.18451345
GTATGCC3400.043.6451642
TAAGACA3250.043.4124531
AAAGGGG3700.043.1247668
CCTAAGA3350.043.02313629
AAGACAT3300.042.8461332
Produced by FastQC (version 0.11.5)