FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990716
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34110.3442964482253239No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTCTTCATTTGCAGAAACA26610.2685936231977681No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25080.2531502468921467No Hit
GTGTTGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCCACTGAACCT24590.2482043289903464No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23020.232357204284578No Hit
GTGTATTACTGTGCGAAGGCGGGGGTCGTTCGAGGTTCGGGGAGTTATTGGTCAAGGAACGCTGGTGCGAATTA22450.22660378958248378No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20370.20560887277484163No Hit
CTCCAGCCCCGTCCCTGGAGCCTGGCGGACCCAGCCCATCCAATAGTTTC18710.1888533141687426No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18220.1839073962669423No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18170.18340271076675857No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17310.17472212016359884No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17040.17199681846260684No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG17010.17169400716249664No Hit
CCATCAACACTCTGCAACCTGGAGATTTTGCAACTTACTACTGTCAACAG16480.16634434086054933No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15700.15847124705768353No Hit
GTAGGAGACAGAGTCACAATCACTTGCCGGGCAAGTCAGGGCATTAGCAACTATTTAAATTGGTATCAGCAGAAA15500.15645250505694872No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15340.15483751145636085No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15250.15392907755603016No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15180.15322251785577298No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14960.1510019016549647No Hit
GAGTTCACTCTCACCATCAACACTCTGCAACCTGGAGATTTTGCAACTTACTACTGTCAACAGAGTTTCAGTGGC14830.14968971935448705No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCCTGGCCGAACGTC14500.1463587950532746No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT14410.14545036115294394No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCTCA13900.14030256905107014No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13850.13979788355088643No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC13840.13969694645084968No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACAATCACTTGCCGGGCAAGTCAGGGCATTAGCAA13670.1379810157502251No Hit
GGGCTGGAGTGGGTGGCCAACATAAAACAAGATGGAAGTGAGAAACACTA13460.13586133664945352No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13420.13545758824930657No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13250.13374165754868195No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13120.1324294752482043No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCCTGGCCGAACGTCCAAAGGCCA12930.13051167034750624No Hit
GATCTATGCAGCATCCACTTTGCAAAGTGAGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCA12930.13051167034750624No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCTCACTTTGCAAAGT12800.1291994880470286No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT12590.12707980894625703No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT12450.12566668954574267No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12010.12122545714412607No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11950.12061983454390562No Hit
CTCCTGTGCAGCCTCTGGATTCACGTTTAGAAACTATTGGATGGGCTGGGTCCGCCAGGCTCCAGGGACGGGG11880.11991327484364843No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11810.11920671514339125No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTCTTCATTTGCAGAAA11800.1191057780433545No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11720.11829828124306058No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11620.11728891024269317No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11350.11456360854170115No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11300.11405892304151746No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11220.11325142624122352No Hit
ACTTACTACTGTCAACAGAGTTTCAGTGGCCTTTGGACGTTCGGCCAGGGGACCAAGGTGGAAGTCAAACGAA11030.11133362134052543No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11020.1112326842404887No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC10880.1098195648399743No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCAGTGAGC10640.10739707443909252No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT10570.10669051473883534No Hit
GTAGTAAGTTGCAAAATCTCCAGGTTGCAGAGTGTTGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCCA10520.10618582923865164No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10380.10477270983813727No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10310.10406615013788006No Hit
GTATTACTGTGCGAAGGCGGGGGTCGTTCGAGGTTCGGGGAGTTATTGGTCAAGGAACGCTGGTGCGAATTACTA10200.10295584203747592No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10150.10245115653729221No Hit
GCGTAGTAGTAGTAATTCGCACCAGCGTTCCTTGACCAATAACTCCCCGAACCTCGAACGACCCCCGCCTTCGCA10130.10224928233721874No Hit
CTCCTGATCTATGCAGCATCCACTTTGCAAAGTGAGGTCCCATCAAGGTT10090.10184553393707177No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9930.10023054033648392No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG100.0068749213146.4988370
GCTAGCG200.00798720850.6881734
GTGGTAT2050.042.2668951
GTATCAA18400.041.113921
TTAGTTC350.001343664439.45265642
CGACGCC556.437165E-638.40332848
CCGACGC556.611377E-638.25422747
TTATATA350.001604088138.055872
TATCAAC21000.035.835952
TCAACGC21950.034.1194734
ATCAACG22050.034.115693
TCTGGAG10750.033.38810770
AACGCAG22700.033.285446
CAACGCA22650.033.2119645
AGAGTAC23600.032.01769611
CGCAGAG23900.031.6126088
TGCACAG9600.028.23154670
AGTACGG24450.027.90944713
GAGTACG24750.027.57115212
TAACGGC500.00836878427.15757436
Produced by FastQC (version 0.11.5)