FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972678
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13830.1421847723501508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13050.038.8227121
GTATCAA28350.037.794271
ATCAACG32500.032.7188343
TCAACGC32550.032.6652154
TATCAAC33000.032.436992
CAACGCA33050.032.2744835
AACGCAG34950.030.2264696
CGCAGAG36550.028.8097468
ACGCAGA36650.028.6378577
AGAGTAC37550.027.86471211
CAGAGTA39600.026.50448210
GCAGAGT40550.025.9678489
AGTACGG31100.024.63189113
TGACGCG700.001406143224.42013510
GAGTACG31600.024.35036812
GTGGTAT4750.023.7823621
TACGGGC6250.022.4389915
GTACGGG34550.022.17226814
TACGGGA10050.021.78278215
TGGTATC5100.021.4580352
Produced by FastQC (version 0.11.5)