FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426957
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT11790.2761402202095293No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10700.25061071723850414No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA7860.1840934801396862No Hit
GTTATTATACTGCTGACAGTAATAAAGTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT7560.17706701143206457No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7160.1676983864885691No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6380.14942956784875291No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6180.1447452553770052No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6010.1407635897760196No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC5870.13748457104579617No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5670.13280025857404842No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATATCAGTGGCCCTGGT5500.12881859297306286No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCACTTTATTACTGTCAGCAGTATAATAACTGG5430.12717908360795116No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT5350.12530535861925207No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5170.1210894773946791No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4690.10984712746248451No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT4680.10961291183889713No Hit
CTGATATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT4680.10961291183889713No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC4660.10914448059172235No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG4650.10891026496813497No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4510.10563124623791155No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4480.10492859936714939No Hit
GGTTTATGCCGGTACCAGGCTAAGTTGCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC4470.104694383743562No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4460.10446016811997462No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4360.10211801188410073No Hit
TTATTACTGTCAGCAGTATAATAACTGGCCTCAGACGTTCGGCCAAGGGA4330.10141536501333857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12350.041.473081
TCGGTTT806.465778E-541.27034870
GGTATCA4700.039.495071
TATCAAC14050.036.1721422
ATCAACG14150.035.91233
TCAACGC14150.035.669654
CAACGCA14300.035.295495
AACGCAG14500.034.808666
CTCGCTA400.002645536334.36324723
CGCAGAG15050.033.08038
ACGCAGA15550.032.899847
AGAGTAC15250.032.87160511
TATTACC553.344043E-431.217362
GCAGAGT16100.030.922899
AGTACGG14450.030.8896813
GAGTACG14800.030.6231712
GCATCGA450.004723610430.52721618
TATTAGC450.004726318630.523642
CAGAGTA16800.029.63443610
ATTACCC703.9732087E-529.4300613
Produced by FastQC (version 0.11.5)