FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800284
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8700.047.0053861
GTATCAA22550.041.6040881
TATCAAC26700.035.5214422
TCAACGC26800.034.9976774
ATCAACG26950.034.6775673
CAACGCA27600.033.9832465
AACGCAG28750.032.860876
ACGCAGA31100.030.3778157
CGCAGAG31300.029.9641918
AGAGTAC31750.029.75590511
CAGAGTA32850.028.75951210
GCAGAGT33950.027.6253059
AGTACGG24750.027.06721713
GAGTACG25100.026.96352812
CGAATTA2300.026.88616815
CGACTTA658.0886343E-426.84761450
GTGGTAT3050.025.9148031
TGGTATC3250.024.3185252
GTACGGG28400.023.46753914
TCGTATG750.001940334923.10513740
Produced by FastQC (version 0.11.5)