FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1351365
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34750.037.72151
GGTATCA15600.036.6384351
ATCAACG37850.034.3569453
TCAACGC38000.034.221324
CAACGCA38600.033.6893845
TATCAAC38950.033.6527252
AACGCAG40900.031.9610316
ACGCAGA42850.030.4267047
CGCAGAG44300.029.4307948
AGAGTAC44750.029.0583711
CAGAGTA46600.028.12506910
GTGGTAT5400.026.619541
GCAGAGT49400.026.5309359
AGTACGG35900.025.64121413
GAGTACG36900.024.9463312
TACGGGG15900.023.67425215
GTACGGG39450.023.073614
TGGTATC7000.021.0232282
GTATCGA1004.5780293E-420.53355416
TCGTATG2051.6716513E-920.26140440
Produced by FastQC (version 0.11.5)