FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1406652
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTGGCTCATTTTACACTTTTGGCCAGGGGA38830.27604553222829814No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31230.22201653287380246No Hit
GTAATATACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA29820.21199273167777105No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA29480.2095756448645436No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21770.15476464683518026No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC21340.1517077429243338No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20650.1468024785092546No Hit
CTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20590.14637593377750857No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT19880.1413284877851807No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19130.13599667863835546No Hit
GTGTATTACTGTCAGCAGTATGGTGGCTCATTTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA18840.13393504576824972No Hit
GTATATTACTGTGCGAGAACCGGTTTTTGGACTGGTTATTTTGACTTCTGGGGCCAGGGAGCCCAGGTCACCGT18000.12796341952380547No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17780.12639942217407005No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT17660.12554633271057802No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17570.12490651561295898No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16810.11950361567750944No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16480.11715761965290633No Hit
CTCCTGCGGGGCCAGTCAGAGTCTTACCGGTCCGAGTCTCAGCGGCGCCTACTTAGCCTGGTACCAGCAGAAAC15860.11274999075819747No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG15510.1102618131563457No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGAGTCTTACCGGTCCGAGTCTCAGCGG15390.10940872369285368No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT15370.10926654211560499No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15190.10798690792036694No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC15180.10791581713174261No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15010.10670727372512889No Hit
GAGTCTTACCGGTCCGAGTCTCAGCGGCGCCTACTTAGCCTGGTACCAGCAGAAACCTGGCCTGGCGCCCAGGCT15010.10670727372512889No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14700.10450345927777446No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14670.10429018691190145No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14500.10308164350528773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)