FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009535
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26850.26596403294586124No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23590.2336719380704978No Hit
GTCTCAGTCAGGACACAGCATGGACTTGAGGGTCCCCGCTCAGCTCCTGG21860.2165353355752896No Hit
GAGTTACAGGACCCCGGTAACTTTCGGCCCTGGGACCAAAGTGGATATCAAGCGAACTGTGGCTGCACCAT19750.19563462386148078No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19490.19305918071191192No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19370.19187051464288013No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17570.1740405236074034No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15630.1548237554913896No Hit
GCATGGACTTGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG15560.15413036695112106No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15470.15323886739934722No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15100.14957381368649925No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14990.14848420312322008No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14650.14511631592763002No Hit
GAATAGATCAGGAGGTTAGGGGCTTTCTCTGGTTTTTGTTGATACCAATT14630.14491820491612475No Hit
GGACACAGCATGGACTTGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14310.14174842873204No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14210.14075787367451353No Hit
GATCTATTCTGCATCCACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGTAGTGGATCTGGGACAGATTTCA13960.13828148603069731No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC13700.13570604288112845No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13610.1348145433293546No Hit
GTGCAGCCACAGTTCGCTTGATATCCACTTTGGTCCCAGGGCCGAAAGTT13500.13372493276607547No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13470.13342776624881753No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13380.1325362666970437No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13010.12887121298419568No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12880.12758349140941128No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATATCCACTTTGGTCCCAGGGCCGAAAGTTACCGGGGTC12060.11946093993769409No Hit
GTCTCCATCCTCCCATTCTGCATTTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGCAC12010.11896566240893085No Hit
CAGCATGGACTTGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT11900.11787605184565171No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11830.11718266330538317No Hit
GTCAGGACACAGCATGGACTTGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11170.11064499992570838No Hit
GTAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCT10890.10787144576463421No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10800.10697994621286037No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10540.10440450306329152No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10480.10381017002877563No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC10130.10034322732743292No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)