FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009535
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24450.24219071156522556No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20900.20702600702303536No Hit
GTCTCAGTCAGGACACAGCATGGACTTGAGGGTCCCCGCTCAGCTCCTGG20110.1992006220685761No Hit
GAGTTACAGGACCCCGGTAACTTTCGGCCCTGGGACCAAAGTGGATATCAAGCGAACTGTGGCTGCACCAT19610.1942478467809437No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19470.19286106970040662No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18600.1842432406999262No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18160.17988479844680969No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15560.15413036695112106No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15370.15224831234182074No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15220.150762479755531No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14910.14769175907719892No Hit
GAATAGATCAGGAGGTTAGGGGCTTTCTCTGGTTTTTGTTGATACCAATT14600.1446210383988668No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14500.14363048334134032No Hit
GATCTATTCTGCATCCACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGTAGTGGATCTGGGACAGATTTCA14380.14244181727230854No Hit
GCATGGACTTGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14330.1419465397435453No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13930.13798431951343937No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13930.13798431951343937No Hit
GGACACAGCATGGACTTGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13890.13758809749042877No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13100.12976271253596952No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13010.12887121298419568No Hit
GTGCAGCCACAGTTCGCTTGATATCCACTTTGGTCCCAGGGCCGAAAGTT12990.12867310197269038No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12910.1278806579266692No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC12670.12550332578860565No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12190.12074866151247851No Hit
GTCTCCATCCTCCCATTCTGCATTTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGCAC11810.11698455229387787No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATATCCACTTTGGTCCCAGGGCCGAAAGTTACCGGGGTC11590.11480533116731961No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11560.11450816465006165No Hit
GTCAGGACACAGCATGGACTTGAGGGTCCCCGCTCAGCTCCTGGGGCTCC10700.1059893911553339No Hit
GTAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCT10470.10371111452302298No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10380.10281961497124914No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10380.10281961497124914No Hit
CAGCATGGACTTGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT10190.10093756036194883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3400.037.8659741
ATCAACG28700.033.1356353
TCAACGC28900.032.9063224
CAACGCA29300.032.3431855
AACGCAG30300.031.2757556
ATAGCGG952.0411608E-730.5859463
AGAGTAC31250.030.32797611
CGCAGAG31550.030.1424038
ACGCAGA31750.029.8474147
GTATCAA33750.028.6098481
CGCTAAT905.175809E-627.84681758
TAGAGCG500.00849284127.0758675
AGTACGG31050.026.92593413
GGTAACG550.0094096426.5123964
TATCAAC37050.026.4051172
TACGGGT2700.026.33158715
CAGAGTA36950.026.19524610
GAGTACG32000.026.02079412
TGTTAGA10350.025.0620870
GTATACT700.001491758524.1736834
Produced by FastQC (version 0.11.5)