FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385801
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACC200.00680490952.77779856
TCAATCG200.006862364752.66572654
CGTATGC506.8137524E-848.40309541
GGTATCA4150.039.672531
GGATAGT350.001385706439.2002376
GTATCAA9650.037.676911
TCGTATG655.3704207E-737.21369640
CTCGTAT753.9659426E-836.84484539
CTTATAC807.444032E-834.300211
TCTCGTA958.682036E-932.71549238
TGGTATC1600.032.1564452
TATCAAC11800.031.684092
GTGGTAT1650.031.1820071
ACGTAGT553.3657358E-431.1820051
GATAGTC553.368296E-431.1779637
CCCACGA1001.5324986E-830.86218319
CAACGCA12000.030.3024445
ATCAACG12000.030.2985173
TCAACGC12000.030.2945884
TTAACGG802.7910119E-630.13771835
Produced by FastQC (version 0.11.5)