FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385801
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5610.1454117537279582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9050.038.613621
GGTATCA4050.033.837161
TATCAAC10300.033.560032
ATCAACG10550.033.0805933
AGCGGTA450.003605504332.2581267
TCAACGC10800.031.9938774
CAACGCA10900.031.6962495
AACGCAG11200.030.8472446
GTGGTAT2000.030.8341121
ACGCAGA11350.030.740957
CGCAGAG11900.029.03278
AGAGTAC12450.027.482511
CAGAGTA12900.026.51693310
GCATAAG801.0009275E-425.6950931
CCACACG801.0106846E-425.6584594
GCAGAGT13350.025.6231049
TGGTATC2150.025.4694582
GTGTAAG700.001387510624.4715181
TATACAG700.001400152224.433465
AGTACGG11750.023.87819913
Produced by FastQC (version 0.11.5)