FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575896
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAC200.006549877653.29338562
GTATCAA15750.038.485161
GGTATCA7100.036.6569061
TCGTATG905.0240487E-934.6089740
TATCAAC18250.033.0227052
TCAACGC18300.032.9267584
CAACGCA18300.032.7396745
ATCAACG18550.032.485823
AACGCAG18800.032.233166
TAGGGCG450.00446275430.8855243
CTCGTAT901.9588515E-730.72577539
ACGCAGA19900.030.454077
CGCCTAG450.004793402730.43759519
TACGGGT2950.030.17440615
CTTATAC1251.364242E-1030.1358341
CGTATGC1052.2313543E-829.69871941
AGAGTAC20600.029.086811
CAGAGTA20900.028.66928510
CGCAGAG21250.028.3582128
GCAGAGT22100.027.1125839
Produced by FastQC (version 0.11.5)